@article{article_1590748, title={In Vitro and Bioinformatic Studies on The Phytochemical Constituent of Selected Zingiberaceae Plants Targeting İnhibitor Lipoxygenase (LOX)}, journal={Fabad Eczacılık Bilimler Dergisi}, volume={50}, pages={417–428}, year={2025}, DOI={10.55262/fabadeczacilik.1590748}, author={Choironi, Nur Amalia and Suganda, Asep Gana and Anggadiredja, Kusnandar}, keywords={Lipoksijenaz, Zingiberaceae, Curcuma zedoaria}, abstract={Lipoxygenase (LOX) is an enzyme essential for the formation of leukotriene, an endogenous substance involved in inflammatory pathological conditions, including asthma and rheumatoid arthritis. Plants that belong to the Zingiberaceae family have been empirically used as drugs in Indonesia. This study aimed to screen the LOX-inhibiting activity of 18 plants from the Zingiberaceae family. The highest activity has been identified for their compound to continue with bioinformatics studies. Bioinformatics analysis to reveal and confirm the gene target of the active compound was acquired from the STITCH, STRING, and Swiss Target Prediction databases. The research result showed that the ethanol extract of Curcuma zedoaria at 100 ppm had the highest inhibiting activity compared to other extracts with a value of 54.8% and the IC50 value is 86.03 ppm. The further test also showed that the essential oil of C. zedoaria (EOCZ) in the selected plants was better than the extracts and their fractions. The bioinformatics study showed that the potential target of EOCZ is MAPK3, MPO, HMOX1, ACE, PARP1, PPARG, ALOX 5 (5-LOX), PTGS1, PTGES, ESR, and NOS2. The further test also showed that the essential oil content of C. zedoaria was better than the extracts and their fractions. The bioinformatics study showed that one of the potential targets of EOCZ is 5-LOX. 5-LOX is essential in inflammatory reactions and might be the potential as an adjunct therapy for this disease for this particular respiratory disorder.}, number={2}, publisher={FABAD Ankara Eczacılık Bilimleri Derneği}