TY  - JOUR
T1  - İndoksakarb ve Abamektin İnsektisitlerinin Apis mellifera Üzerindeki Toksik Etkilerinin Moleküler Kenetlenme Yöntemi ile İncelenmesi
AU  - Sugeçti, Serkan
PY  - 2025
DA  - May
Y2  - 2025
DO  - 10.63716/guffd.1595098
JF  - Gazi Üniversitesi Fen Fakültesi Dergisi
JO  - GÜFFD
PB  - Gazi Üniversitesi
WT  - DergiPark
SN  - 2757-5543
SP  - 131
EP  - 138
VL  - 6
IS  - 1
LA  - tr
AB  - İnsektisitler, hedef olmayan organizmalar üzerinde önemli etkilere yol açarak ekosistem dengesi ve biyolojik çeşitlilik açısından ciddi riskler oluşturmaktadır. Bu çalışmada, yaygın olarak kullanılan iki insektisit olan indoksakarb ve abamektinin, ekolojik ve tarımsal açıdan kritik bir tür olan Apis mellifera üzerindeki etkileri incelendi. Bu insektisitlerin A. mellifera’nın detoksifikasyon enzimi glutatyon-S-transferaz (GST) ve feromon bağlayıcı protein ile olan bağlanma afiniteleri değerlendirildi. Elde edilen bulgular, abamektinin feromon bağlayıcı protein ile daha yüksek bir bağlanma afinitesi (-12.7 kcal/mol) gösterdiğini, indoksakarbın ise nispeten düşük bir bağlanma enerjisine (-10.6 kcal/mol) sahip olduğunu ortaya koydu. GST ile bağlanma enerjileri indoksakarb için -9.3 kcal/mol, abamektin için ise -8.4 kcal/mol olarak belirlendi. Her iki insektisit, proteinlerle hidrofobik etkileşimler ve hidrojen bağları gibi spesifik bağlanmalar sergileyerek, feromon sinyalizasyonu ve detoksifikasyon mekanizmalarını potansiyel olarak bozabileceğini gösterdi. Bu çalışma, insektisitlerin biyotransformasyonunda GST'nin kritik rolünü ve feromon bağlayıcı proteinlerin insektisitlerden olumsuz etkilendiğini gösterdi. Bu çalışmada ayrıca, bu bileşiklerin bal arıları üzerindeki ekolojik riskleri belirlendi.
KW  - Apis mellifera
KW  - Ekolojik riskler
KW  - Detoksifikasyon
KW  - Feromon bağlayıcı protein
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UR  - https://doi.org/10.63716/guffd.1595098
L1  - https://dergipark.org.tr/tr/download/article-file/4411132
ER  -