TY  - JOUR
T1  - Determining the Risk Factors for Nosocomial Multidrug-Resistant Acinetobacter Infections in Patients in Intensive Care Units and Genotyping of Isolates
TT  - Yoğun Bakım Ünitesinde Nosokomiyal Çoklu İlaç Dirençli Acinetobacter Enfeksiyonları için Risk Faktörlerinin Belirlenmesi ve İzolatların Genotiplendirilmesi
AU  - Elmas Dal, Şirvan
AU  - Memişoğlu, Funda
AU  - Otlu, Barış
AU  - Kuzucu, Çiğdem
AU  - Ersoy, Yasemin
PY  - 2025
DA  - September
Y2  - 2025
DO  - 10.26453/otjhs.1604466
JF  - Online Turkish Journal of Health Sciences
JO  - OTSBD
PB  - Oğuz KARABAY
WT  - DergiPark
SN  - 2459-1467
SP  - 203
EP  - 210
VL  - 10
IS  - 3
LA  - en
AB  - Objective: This study aimed to identify the risk factors for hospital-acquired Acinetobacter baumannii infections in an intensive care unit (ICU), delineate the antibiotic resistance profiles of isolates, and elucidate the clonal relationships among strains through genotypic analysis.Materials and Methods: This prospective case-control study was conducted between April 2011 and March 2012 and included 213 patients. The antibiotic susceptibility of Acinetobacter strains was evaluated using the Kirby–Bauer disk diffusion method. The DiversiLab system was used to determine the clonal relationships among A. baumannii strains.Results: The key independent risk factors for hospital-acquired A. baumannii infections included age, mechanical ventilator use, tracheotomy, percutaneous endoscopic gastrostomy (PEG), carbapenem administration, and nonuse of cephalosporins. High multi-antibiotic resistance was observed in 94% of the isolates. Furthermore, a substantial clonal closeness with an 86% clustering rate was observed among the isolates, with the largest cluster (P5) comprising 24 isolates persisting for approximately 14 months in the hospital setting.Conclusions: The findings indicate the need for targeted preventive measures against specific risk factors for A. baumannii infections in ICUs. The genotypic analysis revealed significant clonal spread, necessitating enhanced infection control strategies.
KW  - Acinetobacter infections
KW  - genotype
KW  - intensive care units
N2  - Amaç: Bu çalışma, Yoğun Bakım Ünitesi 'nde (YBÜ) hastane kaynaklı Acinetobacter baumannii enfeksiyonları ile ilişkili risk faktörlerini belirlemeyi, izolatların antibiyotik direnç profillerini çizmeyi ve genotipik analiz yoluyla suşlar arasındaki klonal ilişkileri aydınlatmayı amaçlamaktadır.Materyal ve Metot: Bu çalışma Nisan 2011 ile Mart 2012 tarihleri ​​arasında prospektif vaka-kontrol çalışması olarak yürütülmüş olup 213 hastayı kapsamaktadır. Acinetobacter suşlarının antibiyotik duyarlılıkları, Kirby–Bauer disk difüzyon yöntemi kullanılarak değerlendirilmiştir. A. baumannii suşları arasındaki klonal ilişkilerin belirlenmesi için DiversiLab sistemi kullanılmıştır.Bulgular: Hastane kaynaklı Acinetobacter enfeksiyonu için bağımsız anahtar risk faktörleri yaş, mekanik ventilatör kullanımı, trakeotomi, perkütan endoskopik gastrostomi (PEG) uygulaması, karbapenem verilmesi ve sefalosporin kullanılmamasıdır. İzolatların %94'ünde yüksek çoklu antibiyotik direnci gözlemlenmiştir. Ayrıca izolatlar arasında %86 oranında belirgin bir klonal yakınlık gözlenmiş, en büyük kümeyi (P5) yaklaşık 14 ay boyunca hastane ortamında süregelen 24 izolat oluşturmuştur.Sonuç: Bulgular, YBÜ'lerinde A. baumannii enfeksiyonları için belirli risk faktörlerine karşı hedeflenmiş önleyici tedbirlerin gerekliliğini vurgulamaktadır. Genotipik inceleme, artırılmış enfeksiyon kontrol stratejilerini gerektiren önemli bir klonal yayılımı ortaya koymaktadır.
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UR  - https://doi.org/10.26453/otjhs.1604466
L1  - https://dergipark.org.tr/tr/download/article-file/4454204
ER  -