@article{article_1610557, title={Development of A Rapid and Sensitive LC-MS Method for Determination of Aprepitant Levels in the Plasma of Subacute Sclerosing Panencephalitis Patients}, journal={Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi}, volume={25}, pages={1301–1307}, year={2025}, author={Arıöz, Filiz and Kunal Şalli, Gizem and Tezcan, Songul and Arman Kandırmaz, Emine and Sancar, Mesut and Konuşkan, Bahadır and Öncel, İbrahim Halil and Anlar, Banu}, keywords={Aprepitant, miktar tayini, LC-MS, Subakut sklerozan panensefalit, İnsan plazması}, abstract={In this study, a sensitive, selective, rapid, and validated Liquid Chromatography-Mass Spectrometry (LC-MS) method has been developed for routine monitoring of aprepitant concentrations in the blood of patients with subacute sclerosing panencephalitis (SSPE). The developed method was effectively used to detect and monitor potential drug-induced toxicity in SSPE patients. The analyte was extracted from human plasma using liquid-liquid extraction. Chromatographic separation was carried out on a C18 (50 mm x 2.1 mm x 3 µm) column with acetonitrile/0.2% formic acid- water (50/50, v/v)) isocratic mobile phase at a flow rate of 0.40 mL/min. It was studied in mass spectrometry in positive mode by electrospray ionisation technique and aprepitant was detected at 535.20 m/z. The retention time for the aprepitant was 2.70 min, and the total retention time was 4.8 min. The linear concentration range was 0.0125-3.00 µg/mL (r=0.9976), LOD and LOQ values were 0.01 µg/mL and 0.03 µg/mL, respectively. The developed method was successfully used for the determination of aprepitant concentration in plasma samples from SSPE patients. The developed and validated method is selective, sensitive and rapid. It can be safely and routinely used for aprepitant detection in preclinical and clinical studies.}, number={6}, publisher={Afyon Kocatepe Üniversitesi}, organization={TUBITAK}