TY - JOUR T1 - Development of A Rapid and Sensitive LC-MS Method for Determination of Aprepitant Levels in the Plasma of Subacute Sclerosing Panencephalitis Patients TT - Subakut Sklerozan Panensefalitli Hastaların Plazmasında Aprepitant Tayini İçin Hassas ve Hızlı Bir LC-MS Yönteminin Geliştirilmesi AU - Arıöz, Filiz AU - Kunal Şalli, Gizem AU - Tezcan, Songul AU - Arman Kandırmaz, Emine AU - Sancar, Mesut AU - Konuşkan, Bahadır AU - Öncel, İbrahim Halil AU - Anlar, Banu PY - 2025 DA - November Y2 - 2025 JF - Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi PB - Afyon Kocatepe Üniversitesi WT - DergiPark SN - 2149-3367 SP - 1301 EP - 1307 VL - 25 IS - 6 LA - en AB - In this study, a sensitive, selective, rapid, and validated Liquid Chromatography-Mass Spectrometry (LC-MS) method has been developed for routine monitoring of aprepitant concentrations in the blood of patients with subacute sclerosing panencephalitis (SSPE). The developed method was effectively used to detect and monitor potential drug-induced toxicity in SSPE patients. The analyte was extracted from human plasma using liquid-liquid extraction. Chromatographic separation was carried out on a C18 (50 mm x 2.1 mm x 3 µm) column with acetonitrile/0.2% formic acid- water (50/50, v/v)) isocratic mobile phase at a flow rate of 0.40 mL/min. It was studied in mass spectrometry in positive mode by electrospray ionisation technique and aprepitant was detected at 535.20 m/z. The retention time for the aprepitant was 2.70 min, and the total retention time was 4.8 min. The linear concentration range was 0.0125-3.00 µg/mL (r=0.9976), LOD and LOQ values were 0.01 µg/mL and 0.03 µg/mL, respectively. The developed method was successfully used for the determination of aprepitant concentration in plasma samples from SSPE patients. The developed and validated method is selective, sensitive and rapid. It can be safely and routinely used for aprepitant detection in preclinical and clinical studies. KW - Aprepitant KW - Determination KW - LC-MS KW - Subacute Sclerosing Panencephalitis KW - Human plasma N2 - Bu çalışmada, Subakut Sklerozan Panensefalit (SSPE) hastalarının kanındaki aprepitant düzeylerinin rutin olarak izlenmesi için hassas, seçici, hızlı ve doğrulanmış bir Sıvı Kromatografisi-Kütle Spektrometrisi (LC-MS) yöntemi geliştirilmiştir. Geliştirilen bu yöntem, SSPE hastalarında ilaca bağlı potansiyel toksisitenin tespit edilmesi ve izlenmesinde etkili bir şekilde kullanılmıştır. Aprepitant, insan plazmasından sıvı-sıvı ekstraksiyonu yöntemi kullanılarak ekstrakta edilmiştir. Kromatografik analiz için en uygun koşullar, C18 (50 mm x 2,1 mm x 3 µm) kolonu, asetonitril/su (50/50 (%0,2 formik asit) v/v) izokrotik mobil fazı ve 0,40 mL/dakika akış hızı olarak bulunmuştur. Kütle spektrometresinde, pozitif modda, elektrosprey iyonizasyon tekniği ile çalışılmış ve aprepitant 535.20 m/z değerinde tespit edilmiştir. Aprepitant için alıkonma zamanı 2,70 dakika, toplam analiz süresi 4,8 dakika, doğrusal konsantrasyon aralığı 0,0125-3,00 µg/mL (r=0,9976), LOD ve LOQ değerleri sırasıyla 0,01 μg/mL ve 0,03 μg/mL olarak bulunmuştur. Geliştirilen yöntem, SSPE hastalarından alınan plazma örneklerinde aprepitantın konsantrasyonunun tespiti için başarıyla kullanılmıştır. Geliştirilen ve valide edilen bu yöntem seçici, duyarlı ve hızlı bir yöntemdir. Preklinik ve klinik çalışmalarda, aprepitant tespiti için güvenle ve rutin olarak kullanılabilir. CR - Adel, N. 2017. Overview of chemotherapy-induced nausea and vomiting and evidence-based therapies, The American Journal of Managed Care, 16, 259-265. CR - Chaitanya, K. K., Sankar, D. G., Israel, D. S., and Narasimha, K., 2013. 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