TY  - JOUR
T1  - Oxidative stress in the cerebellum of pinealectomized rats and its correlation with GFAP expression
TT  - Pinealektomize sıçanların serebellumundaki oksidatif stres ve GFAP ekspresyonu ile korelasyonu
AU  - Başak, Feyza
AU  - Demir, Mehmet
AU  - Kuşat, Tansu
AU  - Weli, Sarab Hayder Weli
PY  - 2025
DA  - March
Y2  - 2025
DO  - 10.32322/jhsm.1624240
JF  - Journal of Health Sciences and Medicine
JO  - J Health Sci Med /JHSM /jhsm
PB  - MediHealth Academy Yayıncılık
WT  - DergiPark
SN  - 2636-8579
SP  - 338
EP  - 344
VL  - 8
IS  - 2
LA  - en
AB  - Aims: This study aims to investigate the results of the lack of melatonin in the cerebellar tissue of pinealectomized Wistar albino rats using immunohistochemistry and biochemistry. Methods: Control, pinealectomy, and sham pinealectomy groups were designed for the experiment (n=8). Pinealectomy and sham pinealectomy surgery were employed. At the end of 30 days, cerebellum tissue was used for histopathological, glial fibrillary acidic protein (GFAP) immunostaining, and biochemical (oxidative stress markers) analyses. Purkinje cell counts and cerebellar layer thickness in the cerebellum were also measured within the scope of histometrical analyses.Results: The study revealed that melatonin deficiency (pinealectomy) adversely impacts the overall histological structure of the cerebellum, leading to heightened immunoreactivity to GFAP antibody, elevated malondialdehyde levels, and reduced glutathione and superoxide dismutase levels in comparison to control and sham pinealectomy groups (p
KW  - Cerebellum
KW  - GFAP
KW  - oxidative stress
KW  - pinealectomy
N2  - Amaçlar: Bu çalışmanın amacı, Wistar albino sıçanlarının serebellar dokusunda melatonin eksikliğinin (Pinealektomi) sonuçlarını immünohistokimya ve biyokimya kullanarak araştırmaktır.Gereçler ve Yöntemler: Deney için kontrol, pinealektomi ve sham pinealektomi grupları tasarlandı (n=8). Pinealektomi ve sham pinealektomi ameliyatı yapıldı. 30 günün sonunda, serebellum dokusu histopatolojik, Glial Fibriller Asidik Protein immünoboyama ve biyokimyasal (oksidatif stres belirteçleri) analizler için kullanıldı. Ayrıca, serebellumdaki Purkinje hücre sayıları ve serebellar tabaka kalınlığı histometrik analizler kapsamında ölçüldü. Sonuçlar: Çalışma, melatonin eksikliğinin (pinealektomi) serebellumun genel histolojik yapısını olumsuz yönde etkileyerek Glial Fibriller Asidik Protein antikoruna karşı artan immünoreaktiviteye, yüksek malondialdehit seviyelerine ve kontrol ve sahte pinealektomi gruplarına kıyasla azalmış glutatyon ve süperoksit dismutaz seviyelerine yol açtığını ortaya koydu (P˂ 0,05). Bu çalışma, ilk kez, pinealektomize edilmiş bir sıçan modelinin serebellumundaki oksidan ve antioksidan miktarlarını, GFAP immünoreaktivitesini, Purkinje hücre sayılarını ve serebellar tabaka kalınlığını açıklığa kavuşturdu. Bu çalışma, literatürde bugüne kadar göz ardı edilmiş bir konu olan melatonin ve serebellum arasındaki ilişkiyi açıklamak için yapılan ilk araştırmadır ve böylece önemli bir temel oluşturmuştur.Sonuç: Melatonin eksikliği, serebellumda nörodejenerasyon ve oksidatif stresin bir nedenidir.
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UR  - https://doi.org/10.32322/jhsm.1624240
L1  - https://dergipark.org.tr/tr/download/article-file/4540035
ER  -