TY - JOUR T1 - Determination of physicochemical and sensory properties of beverages produced using protein and prebiotic sources TT - Protein ve prebiyotik kaynaklar kullanılarak üretilen içeceklerin fizikokimyasal ve duyusal özelliklerinin belirlenmesi AU - Özer, Emir Ayşe AU - Özbuldu, Neslihan AU - Özpalas, Beyza PY - 2025 DA - August Y2 - 2025 DO - 10.37908/mkutbd.1626355 JF - Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi JO - MKU. Tar. Bil. Derg. PB - Hatay Mustafa Kemal Üniversitesi WT - DergiPark SN - 2667-7733 SP - 487 EP - 502 VL - 30 IS - 2 LA - en AB - The aim of this study was to determine the physicochemical and sensory properties of protein and prebiotic containing beverages that can be consumed by individuals from all age groups. The study was designed by using the Response Surface Method, and chocolate whey protein isolate (WPI), pea protein isolate (PPI) and inulin were used as independent variables. The maximum and minimum ranges of these variables were determined as 5.0-10.0 g/100 mL for WPI, 2.0-6.0 g/100 mL for PPI and 3.0-7.0 g/100 mL for inulin. As a result of the experimental design, the optimum production conditions were determined as 9.66 g WPI, 2.00 g PPI and 5.00 g inulin. Under these production conditions, the optimum physicochemical responses were determined as follows: the pH value was 7.02, the titration acidity was 0.02, the water-soluble dry matter content was 15.38, the L* value was 46.87, the a* value was 5.79, and the b* value was 8.54. Sensory responses were determined as follows: the taste value was 6.16, the odour value was 6.71, the aftertaste value was 6.50, and the overall acceptability value was 6.56. Under these conditions, the desirability value of the optimised beverage was found to be 97%. With this study, a new functional beverage formulation with high sensory acceptability was developed. KW - Functional beverages KW - Response Surface Method KW - Sensory evaluation KW - Optimisation N2 - Bu çalışmanın amacı, her yaş grubundan bireylerin tüketebileceği protein ve prebiyotik içeren içeceklerin fizikokimyasal ve duyusal özelliklerinin belirlenmesidir. Çalışma, Tepki Yüzey Yöntemi (TYY) kullanılarak tasarlanmış ve bağımsız değişken olarak çikolatalı peynir altı suyu protein izolatı (WPI), bezelye protein izolatı (PPI) ve inülin kullanılmıştır. Bu değişkenlerin maksimum ve minimum aralıkları WPI için 5.0-10.0 g/100 mL, PPI için 2.0-6.0 g/100 mL ve inülin için 3.0-7.0 g/100 mL olarak belirlenmiştir. Deneysel tasarım sonucunda, optimum üretim koşulları 9.66 g WPI, 2.00 g PPI ve 5.00 g inülin olarak belirlenmiştir. Bu üretim koşullarında, optimum fizikokimyasal yanıtlar şu şekilde belirlenmiştir: pH değeri 7.02, titrasyon asitliği 0.02, suda çözünür kuru madde içeriği 15.38, L* değeri 46.87, a* değeri 5.79 ve b* değeri 8.54. Duyusal yanıtlar şu şekilde belirlenmiştir: tat değeri 6.16, koku değeri 6.71, ağızda kalan tat değeri 6.50 ve genel kabul edilebilirlik değeri 6.56. Bu koşullar altında optimize edilmiş içeceğin arzu edilebilirlik değerinin %97 olduğu tespit edilmiştir. Bu çalışma ile, duyusal kabul edilebilirliği yüksek yeni bir fonksiyonel içecek formülasyonu geliştirilmiştir. CR - Ahern, N., Arendt, E.K., & Sahin, A.W. (2023). Protein soft drinks: A retail market analysis and selected product characterization. Beverages, 9 (3), 73. https://doi.org/10.3390/beverages9030073 CR - Akin, Z., & Ozcan, T. (2017). Functional properties of fermented milk produced with plant proteins. LWT - Food Science and Technology, 86, 25-30. https://doi.org/10.1016/j.lwt.2017.07.025 CR - AOAC. (2000). Official methods of analysis : Association of official analytical chemists (17th ed.). Washington, DC, USA. CR - Aribah, S.A., Sanjaya, A.P., Muhammad, D.R.A., & Praseptiangga, D. (2020). Sensorial and physical properties of chocolate beverage prepared using low fat cocoa powder. In AIP Conference Proceedings, 2219 (1), 070007. https://doi.org/10.1063/5.0003435 CR - Birch, C.S., & Bonwick, G.A. (2019). Ensuring the future of functional foods. International Journal of Food Science & Technology, 54 (5),1467-1485. https://doi.org/10.1111/ijfs.14060 CR - Boukid, F., Rosell, C.M., & Castellari, M. (2021). Pea protein ingredients: A mainstream ingredient to (re)formulate innovative foods and beverages. Trends in Food Science & Technology, 110, 729-742. https://doi.org/10.1016/j.tifs.2021.02.040 CR - Bäuerle, L., & Kühn, S. (2022). Development of a protein supplemented fruit smoothie using pea protein isolate as a plant-based protein alternative. Future Foods, 5, 100145. https://doi.org/10.1016/j.fufo.2022.100145 CR - Carvalho, F., Lahlou, R.A., Pires, P., Salgado, M., & Silva, L.R. (2023). Natural functional beverages as an approach to manage diabetes. International Journal of Molecular Sciences, 24 (23), 16977. https://doi.org/10.3390/ijms242316977 CR - Çiftçi, M., & Öncül, N. (2022). Some microbiological properties of commercial probiotic drinks (in Turkish). Academic Journal of Agriculture, 11 (1), 165-178. http://dx.doi.org/10.29278/azd.1002242 CR - Demirbağ, Z., Alan, S., & Öksüztepe, G. (2023). Functional foods and their ımportance in nutrition (in Turkish). Bozok Veterinary Sciences, 4 (2), 54-60. https://doi.org/10.58833/bozokvetsci.1324245 CR - Doğan, M., Aktar, T., Toker, O.S., & Tatlısu, N.B. (2015). Combination of the simple additive (SAW) approach and mixture design to determine optimum cocoa combination of the hot chocolate beverage. International Journal of Food Properties, 18 (8), 1677-1692. https://doi.org/10.1080/10942912.2014.917662 CR - Ersus, S., Tekin, İ., Visali, R., Allahverdiyeva, Z., Askerova, İ., Hasanova, A., Kamalov, R., & Aliyeva, D. (2024). Production of functional apple juice enriched with aromatic plant extracts (in Turkish). Journal of Agriculture Faculty of Ege University, 61 (1), 103-111. https://doi.org/10.20289/zfdergi.1433287 CR - Garay, P.A., Villalva, F.J., Paz, N.F., de Oliveira, E.G., Ibarguren, C., Alcocer, J.C., Curti, C.A., & Ramón, A.N. (2021). Formulation of a protein fortified drink based on goat milk whey for athletes. Small Ruminant Research, 201, 106418. https://doi.org/10.1016/j.smallrumres.2021.106418 CR - Gayathry, K.S., & John, J.A. (2021). Functional beverages: Special focus on anti-diabetic potential. Journal of Food Processing and Preservation, 45 (11), e15974. https://doi.org/10.1111/jfpp.15974 CR - Gimhani, K.H.I., & Liyanage, A.L.C.J. (2019). Development and quality evaluation of ready to drink fruit flavoured whey beverage. International Journal of Scientific and Research Publications, 9 (8), 779-783. http://dx.doi.org/10.29322/IJSRP.9.08.2019.p92107 CR - Hidalgo-Fuentes, B., de Jesús-José, E., Cabrera-Hidalgo, A.J., Sandoval-Castilla, O., Espinosa-Solares, T., González-Reza, R.M., Zambrano-Zaragoza, M.L., Liceaga, A.M., & Aguilar-Toalá, J.E. (2024). Plant-based fermented beverages: Nutritional composition, sensory properties, and health benefits. Foods, 13 (6), 844. https://doi.org/10.3390/foods13060844 CR - King, J., Leong, S.Y., Alpos, M., Johnson, C., McLeod, S., Peng, M., Sutton, K., & Oey, I. (2024).Role of food processing and incorporating legumes in food products to increase protein intake and enhance satiety. Trends in Food Science & Technology, 147, 104466. https://doi.org/10.1016/j.tifs.2024.104466 CR - Lawless, H.T., & Heymann, H. (2010). Sensory evaluation of food: principles and practices (2nd ed.). Springer, Springer New York, USA. CR - Ling, L., Loong, C., & Loke, W.M. (2021). Food additives in commercial cocoa beverage products and their effects on total polyphenol contents, cellular antioxidant and anti-inflammatory activities. Current Research in Nutrition and Food Science Journal, 9 (1), 20-30. https://dx.doi.org/10.12944/CRNFSJ.9.1.03 CR - Liu, Y., Toro-Gipson, R.S.D., & Drake, M. (2021). Sensory properties and consumer acceptance of ready-to-drink vanilla protein beverages. Journal of Sensory Studies, 36 (6), e12704. https://doi.org/10.1111/joss.12704 CR - Lu, Z.X., He, J.F., Zhang, Y.C., & Bing, D.J. (2020). Composition, physicochemical properties of pea protein and its application in functional foods. Critical Reviews in Food Science and Nutrition, 60 (15), 2593-2605. https://doi.org/10.1080/10408398.2019.1651248 CR - Mantzourani, I., Terpou, A., Bekatorou, A., Mallouchos, A., Alexopoulos, A., Kimbaris, A., Bezirtzoglou, E., Koutinas, A.A., & Plessas, S. (2020). Functional pomegranate beverage production by fermentation with a novel synbiotic L. paracasei biocatalyst. Food Chemistry, 308, 125658. https://doi.org/10.1016/j.foodchem.2019.125658 CR - Munekata, P.E.S., Pérez-Álvarez, J.A., Pateiro, M., Viuda-Matos, M., Fernández-López, J., & Lorenzo, J.M. (2021). Satiety from healthier and functional foods. Trends in Food Science & Technology, 113, 397-410. https://doi.org/10.1016/j.tifs.2021.05.025 CR - Myers, R.H., Montgomery, D.C., & Anderson-Cook, C.M. (2016). Response surface methodology: Process and product optimization using designed experiments (4th ed.). John Wiley & Sons, Inc, USA. pp 825. CR - Nam, S.-H., Wagh, A., Martini, S., & Walsh, M.K. (2017). Sensory characterisation of a high-protein beverage. International Journal of Dairy Technology, 70 (3), 432-438. https://doi.org/10.1111/1471-0307.12362 CR - Oba, Ş., & Yılmaz, O. (2022). Effects of different prebiotic agents on the color and sensory properties of chocolate powder drinks (in Turkish). Harran Journal of Agricultural and Food Science, 26 (4), 501-518. https://doi.org/10.29050/harranziraat.1100382 CR - Oba, Ş., & Yılmaz, O. (2023). Optimization of cocoa powder beverages in terms of physical properties of prebiotic combination (in Turkish). Journal of Engineering Sciences and Design, 11 (3), 1054-1065. https://doi.org/10.21923/jesd.1094259 CR - Obayomi, O.V., Olaniran, A.F., & Owa, S.O. (2024). Unveiling the role of functional foods with emphasis on prebiotics and probiotics in human health: A review. Journal of Functional Foods, 119, 106337. https://doi.org/10.1016/j.jff.2024.106337 CR - Oliveira, D.F., Granato, D., & Barana, A.C. (2018). Development and optimization of a mixed beverage made of whey and water-soluble soybean extract flavored with chocolate using a simplex-centroid design. Food Science and Technology, 38 (3), 413-420. https://doi.org/10.1590/1678-457X.07017 CR - Oltman, A.E., Lopetcharat, K., Bastian, E., & Drake, M.A. (2015). Identifying key attributes for protein beverages. Journal of Food Science, 80 (6), 1383-S1390. https://doi.org/10.1111/1750-3841.12877 CR - Pérez-Marroquín, X.A., Estrada-Fernández, A.G., García-Ceja, A., Aguirre-Álvarez, G., & León-López, A. (2023). Agro-food waste as an ingredient in functional beverage processing: sources, functionality, market and regulation. Foods, 12 (8), 1583. https://doi.org/10.3390/foods12081583 CR - Plasek, B., Lakner, Z., Kasza, G., & Temesi, Á. (2019). Consumer evaluation of the role of functional food products in disease prevention and the characteristics of target groups. Nutrients, 12 (1), 69. https://doi.org/10.3390/nu12010069 CR - Pérez-Ramírez, I.F., Cariño-Sarabia, A., Castaño-Tostado, E., Vázquez-Landaverde, P.A., Ramos-Gómez, M., Reynoso-Camacho, R., & Amaya-Llano, S.L. (2021). Chemical and sensorial characterization of Tejate, a Mexican traditional maize-cocoa beverage, and improvement of its nutritional value by protein addition. Journal of Food Science and Technology, 58 (9), 3548-3560. https://doi.org/10.1007/s13197-021-05073-w CR - Quant, A.J.B., Albis, A.R., & Perez, J.A. (2019). Effect of heat treatment on physical properties of whey protein beverages (WPB). Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry, 20, 209-218. CR - Sadler, I., Bauer, A., & Kassam, S. (2024). Dietary habits and self-reported health outcomes in a cross-sectional survey of health-conscious adults eating a plant-based diet. Journal of Human Nutrition and Dietetics, 37 (4), 1061-1074. https://doi.org/10.1111/jhn.13321 CR - Saubenova, M., Oleinikova, Y., Rapoport, A., Maksimovich, S., Yermekbay, Z., & Khamedova, E. (2024). Bioactive peptides derived from whey proteins for health and functional beverages. Fermentation, 10 (7), 359. https://doi.org/10.3390/fermentation10070359 CR - Silva, J.D.C., Anghinoni, I.C.A.B., & Gomes, M.B. (2024). Plant-based food for the prevention of Type 2 diabetes: Scoping review. Nutrients, 16 (11), 1671. https://doi.org/10.3390/nu16111671 CR - Singh, J., Prakash, S., Bhandari, B., & Bansal, N. (2020). Ultra-high temperature (UHT) stability of chocolate flavored high protein beverages. Journal of Food Science, 85 (10), 3012-3019. https://doi.org/10.1111/1750-3841.15397 CR - Tan, M., Nawaz, M.A., & Buckow, R. (2023). Functional and food application of plant proteins – a review. Food Reviews International, 39 (5), 2428-2456. https://doi.org/10.1080/87559129.2021.1955918 CR - Thakur, A., Pandey, P., Dalbhagat, C.G., & Mishra, H.N. (2022). Development of grain-based carbonated beverage premix using maize (Zea mays), Bengal gram (Cicer arietinum), and finger millet (Eleusine coracana). Journal of Food Science and Technology, 59 (4), 1637-1648. https://doi.org/10.1007/s13197-021-05175-5 CR - Tireki, S. (2021). A review on packed non-alcoholic beverages: Ingredients, production, trends and future opportunities for functional product development. Trends in Food Science & Technology, 112, 442-454. https://doi.org/10.1016/j.tifs.2021.03.058 CR - Tjahyo, A.S., Wu, J.Y., Smith, G., Acuin, C., Maier, A.B., Sim, S.Y.J., Taneja, R., Haldar, S., & Henry, C.J. (2024). Shifting trend of protein consumption in southeast asia: toward health, ınnovation, and sustainability. Current Developments in Nutrition, 8 (10), 104443. https://doi.org/10.1016/j.cdnut.2024.104443 CR - Topolska, K., Florkiewicz, A., & Filipiak-Florkiewicz, A. (2021). Functional food—consumer motivations and expectations. International Journal of Environmental Research and Public Health, 18 (10), 5327. https://doi.org/10.3390/ijerph18105327 CR - Trikusuma, M., Paravisini, L., & Peterson, D.G. (2020). Identification of aroma compounds in pea protein UHT beverages. Food Chemistry, 312, 126082. https://doi.org/10.1016/j.foodchem.2019.126082 CR - Vasquez-Orejarena, E., Simons, C.T., Litchfield, J.H., & Alvarez, V.B. (2018). Functional properties of a high protein beverage stabilized with oat-β-glucan. Journal of Food Science, 83 (5), 1360-1365. https://doi.org/10.1111/1750-3841.14119 CR - Wanyonyi, M., Kiluyi, J.W., & Okango, A.A. (2025). Optimization of a functional beverage formulation using a constrained mixture design with multiple responses. Asian Journal of Probability and Statistics, 27 (1), 1-16. https://doi.org/10.9734/ajpas/2025/v27i1699 CR - Yılmaz Ersan, L., & Topçuoğlu, E. (2019). Microbiological and some physico-chemical properties of probiotic yogurt enriched with almond milk (in Turkish). Journal of Agricultural Faculty of Bursa Uludag University, 33 (2), 321-339. CR - Yuan, L., Jiang, Q.Q., Zhai, Y., Zhao, Z., Liu, Y., Hu, F., & Sun, J. (2024). Association between plant-based diet and risk of chronic diseases and all-cause mortality in centenarians in China: A cohort study. Current Developments in Nutrition, 8 (1), 102065. https://doi.org/10.1016/j.cdnut.2023.102065 UR - https://doi.org/10.37908/mkutbd.1626355 L1 - https://dergipark.org.tr/tr/download/article-file/4549607 ER -