@article{article_1634724, title={Temporal regulation of PATL2 transcript levels during oocyte maturation and embryo development: An in silico analysis}, journal={Acta Medica Nicomedia}, volume={8}, pages={263–270}, year={2025}, DOI={10.53446/actamednicomedia.1634724}, author={Berkel, Çağlar}, keywords={PATL2 geni, oosit olgunlaşmasının durması, oosit büyümesi, germinal vezikül durması, sekonder folikül evresi, kadın doğurganlığı, gen regülasyonu}, abstract={Objective: Female infertility is a relatively frequent disorder of the reproductive system, and its molecular etiology and associated cellular mechanisms remain unexplained. Certain mutations in PATL2 gene have been identified to cause female infertility, characterized by oocyte maturation arrest and oocyte meiotic deficiency. PATL2 encodes an RNA-binding protein functioning as a translational repressor in oocyte maturation; therefore, proper temporal control of PATL2 expression was suggested to be essential for normal oocyte maturation. Methods: Transcriptomics data was used to analyze temporal regulation of PATL2 transcript levels during oocyte maturation and embryo development in mice, using R programming language. Results: Based on in silico analysis, mRNA expression of the PATL2 gene in mouse oocytes was found to increase from the primordial follicle stage to the primary follicle stage (p ≤ 0.05). Its expression was reported to stay high in the primary and secondary follicle stages, then to decrease in the antral follicle stages (tertiary follicle stages). Besides, PATL2 expression was shown to be lower in metaphase 2 (MII)-stage oocytes compared to germinal vesicle (GV)-stage oocytes in mice (p ≤ 0.05). Moreover, it was observed that PATL2 transcript levels even change during embryo development. Conclusion: Data in the present study points to the temporal regulation of PATL2 expression at the transcription level during oocyte growth, supporting previous research performed at the protein level. Considering the importance of PATL2 in oocyte maturation arrest and female sterility, and also the number of PATL2 pathogenic variants previously identified, a better mechanistic understanding of PATL2 regulation at the transcriptional level in oocytes might have clinical significance and guide the development of novel treatment strategies.}, number={3}, publisher={Kocaeli Üniversitesi}, organization={None}