TY - JOUR T1 - MODERN EKSTRAKSİYON YÖNTEMLERİNİN BİTKİSEL PROTEİN İZOLATLARININ TEKNOFONKSİYONEL ÖZELLİKLERİNE ETKİSİ TT - EFFECT OF MODERN EXTRACTION METHODS ON TECHNO-FUNCTIONAL PROPERTIES OF PLANT PROTEIN ISOLATES AU - Özdal, Harun Reşit AU - Nakilcioğlu, Emine PY - 2025 DA - June Y2 - 2025 DO - 10.15237/10.15237/gida.GD25026 JF - Gıda JO - GIDA PB - Gıda Teknolojisi Derneği WT - DergiPark SN - 1300-3070 SP - 427 EP - 441 VL - 50 IS - 3 LA - tr AB - Bitkisel protein kaynaklarının önemi; hayvansal proteinlerin sağlık ve çevre üzerindeki etkileriyle olumlu bir alternatif oluşturmaları, insan nüfusunun artmasına bağlı olarak doğru ve dengeli beslenme için gerekli besin öğelerine olan talebin artması, bitki proteinlerinin sağlık üzerine olan olumlu etkileri ve özel beslenme alışkanlıklarının popülerlik kazanması, tarımının ekolojik ve sürdürülebilir olması gibi nedenlerden dolayı artmaktadır. Bitki materyallerinden protein ekstraksiyonunda kullanılan kimyasal ekstraksiyon metotları geleneksel metotlar olup ekonomik ve yüksek verimli olmaları nedeniyle tercih edilmektedirler. Fakat hayvansal proteinlerle kıyaslandığında hali hazırda düşük teknofonksiyonel özellikler sergileyen bitki proteinlerinin bu özelliklerini geliştirmekte yetersiz kalmaktadır. Gıda endüstrisinde proteinlerin teknofonksiyonel özellikleri birçok gıdanın prosesinde önem taşımaktadır. Bitki proteinlerine olan bu ilginin karşılanması ve bitki kaynaklı protein izolatlarının teknofonksiyonel özelliklerini geliştirecek modern ekstraksiyon metotlarında ön işlem uygulanması ve/veya geleneksel metotlarla kombine halde kullanılması, protein izolatlarının teknofonksiyonel özelliklerini geliştirmede oldukça önemlidir. Son zamanlarda gerçekleştirilen, çalışmalar modern ekstraksiyon metotlarının bitkisel protein izolatlarının teknofonksiyonel özelliklerini geliştirmede etkili olduğunu göstermektedir. KW - Bitkisel proteinler KW - modern ekstraksiyon metotları KW - tekno-fonksiyonel özellikler N2 - The importance of plant protein sources is increasing due to several factors: they offer a healthier and more environmentally friendly alternative to animal proteins, the growing human population raises the demand for nutrients essential for a balanced diet, and plant proteins have positive health effects. Additionally, special dietary habits are becoming more popular, and plant-based agriculture is ecological and sustainable. Chemical extraction methods used for extracting proteins from plant materials are traditional, preferred for being economical and efficient. However, they are inadequate for enhancing the techno-functional properties of plant proteins, which are generally lower than those of animal proteins. In the food industry, these properties are crucial in many food processes. To meet the rising interest in plant proteins, applying pre-treatments and/or combining traditional methods with modern extraction techniques is important to improve these properties. Recent studies have shown that modern extraction methods can effectively enhance the techno-functional properties of plant-based protein isolates. CR - Alexandre, E. M., Araújo, P., Duarte, M. F., de Freitas, V., Pintado, M., Saraiva, J. A. (2017). High-pressure assisted extraction of bioactive compounds from industrial fermented fig by-product. Journal of food science and technology, 54: 2519-2531. https://doi.org/10.1007/s13197-017-2697-2 CR - Amagliani, L., Silva, J. V., Saffon, M., Dombrowski, J. (2021). On the foaming properties of plant proteins: Current status and future opportunities. Trends in Food Science & Technology, 118, 261-272. https://doi.org/ 10.1016/j.tifs.2021.10.001 CR - Ampofo, J., Ngadi, M. (2022). Ultrasound-assisted processing: Science, technology and challenges for the plant-based protein industry. Ultrasonics Sonochemistry, 84: 105955. https://doi.org/10.1016/j.ultsonch.2022.105955 CR - Anonymous (2025). Protein Ingredients Market Size | Share and Trends 2025 to 2034. https://www.precedenceresearch.com/protein-ingredients-market. (Accessed: 15 April 2025) CR - Aryee, A. N. A., Agyei, D., Udenigwe, C. C. (2018). Impact of processing on the chemistry and functionality of food proteins. In Proteins in food processing (pp. 27-45). Woodhead Publishing. https://doi.org/10.1016/B978-0-08-100722-8.00003-6 CR - Beniwal, A., Das, M. (2023). Proteın: It’s applıcatıon ın food ındustry. Research Trends in Nutrition Science. sl: Bumi Publishing, 1-25. CR - Bessada, S. M., Barreira, J. C., Oliveira, M. B. P. (2019). Pulses and food security: Dietary protein, digestibility, bioactive and functional properties. Trends in Food Science & Technology, 93: 53-68. https://doi.org/10.1016/j.tifs.2019.08.022 CR - Bose, U., Broadbent, J. A., Byrne, K., Hasan, S., Howitt, C. A., Colgrave, M. L. (2019). Optimisation of protein extraction for in-depth profiling of the cereal grain proteome. Journal of proteomics, 197: 23-33. https://doi.org/10.1016/ j.jprot.2019.02.009 CR - Chen, R., Wang, X. J., Zhang, Y. Y., Xing, Y., Yang, L., Ni, H., Li, H. H. (2019). Simultaneous extraction and separation of oil, proteins, and glucosinolates from Moringa oleifera seeds. Food chemistry, 300: 125162. https://doi.org/10.1016/ j.crfs.2024.100906 CR - De Angelis, D., Latrofa, V., Squeo, G., Pasqualone, A., Summo, C. (2024). Techno-functional, rheological, and chemical properties of plant-based protein ingredients obtained with dry fractionation and wet extraction. Current Research in Food Science, 9: 100906. https://doi.org/10.1016/j.crfs.2024.100906 CR - De Angelis, D., Opaluwa, C., Pasqualone, A., Karbstein, H. P., Summo, C. (2023). Rheological properties of dry-fractionated mung bean protein and structural, textural, and rheological evaluation of meat analogues produced by high-moisture extrusion cooking. Current Research in Food Science, 7: 100552. https://doi.org/10.1016 /j.crfs.2023.100552 CR - Delahaije, R. J., Wierenga, P. A. (2022). Hydrophobicity enhances the formation of protein-stabilized foams. Molecules, 27(7): 2358. https://doi.org/10.3390/molecules27072358 CR - Fan, L., Fan, W., Mei, Y., Liu, L., Li, L., Wang, Z., Yang, L. (2022). Mechanochemical assisted extraction as a green approach in preparation of bioactive components extraction from natural products-A review. Trends in Food Science & Technology, 129: 98-110. https://doi.org/ 10.1016/j.tifs.2022.09.009 CR - Fatima, K., Imran, M., Ahmad, M. H., Khan, M. K., Khalid, W., Al-Farga, A., Eskandrani, A. A. (2023). Ultrasound-assisted extraction of protein from Moringa oleifera seeds and its impact on techno-functional properties. Molecules, 28(6): 2554. https://doi.org/10.3390/ molecules28062554 CR - Gao, K., Rao, J., Chen, B. (2024). Plant protein solubility: A challenge or insurmountable obstacle. Advances in colloid and interface science, 324: 103074. https://doi.org/10.1016/ j.cis.2023.103074 CR - Golberg, A., Sack, M., Teissie, J., Pataro, G., Pliquett, U., Saulis, G., Frey, W. (2016). Energy-efficient biomass processing with pulsed electric fields for bioeconomy and sustainable development. Biotechnology for biofuels, 9: 1-22. https://doi.org/10.1186/s13068-016-0508-z CR - Grossmann, L., McClements, D. J. (2023). Current insights into protein solubility: A review of its importance for alternative proteins. Food Hydrocolloids, 137: 108416. https://doi.org/ 10.1016/j.foodhyd.2022.108416 CR - Grunovaitė, L., Pukalskienė, M., Pukalskas, A., Venskutonis, P. R. (2016). Fractionation of black chokeberry pomace into functional ingredients using high pressure extraction methods and evaluation of their antioxidant capacity and chemical composition. Journal of Functional Foods, 24: 85-96. https://doi.org/10.1016/ j.jff.2016.03.018 CR - Gulzar, S., Tagrida, M., Patil, U., Ma, L., Zhang, B., Benjakul, S. (2023). Mung bean protein isolate treated with high-intensity pulsed electric field: characteristics and its use for encapsulation of Asian seabass oil. Journal of Microencapsulation, 40(5): 330-344. https://doi.org/10.1080/ 02652048.2023.2198030 CR - Gültekin Subaşı, B., Vahapoğlu, B., Capanoglu, E., Mohammadifar, M. A. (2022). A review on protein extracts from sunflower cake: Techno-functional properties and promising modification methods. Critical Reviews in Food Science and Nutrition, 62(24): 6682-6697. https://doi.org/ 10.1080/10408398.2021.1904821 CR - Hadidi, M., Aghababaei, F., McClements, D. J. (2023). Enhanced alkaline extraction techniques for isolating and modifying plant-based proteins. Food Hydrocolloids, 145(5): 109132. https://doi.org/10.1016/j.foodhyd.2023.109132 CR - Henao-Ardila, A., Quintanilla-Carvajal, M. X., Moreno, F. L. (2024). Emulsification and stabilization technologies used for the inclusion of lipophilic functional ingredients in food systems. Heliyon, 10(11): e32150. https://doi.org/10.1016/j.heliyon.2024.e32150 CR - Hussain, M., Qayum, A., Zhang, X., Hao, X., Liu, L., Wang, Y., Li, X. (2021). Improvement in bioactive, functional, structural and digestibility of potato protein and its fraction patatin via ultra-sonication. Lwt, 148: 111747. https://doi.org/ 10.1016/j.lwt.2021.111747 CR - Jahan, K., Fatima, S., Osama, K., Younis, K., Yousuf, O. (2023). Boosting protein yield from mustard (Brassica juncea) meal via microwave-assisted extraction and advanced optimization methods. Biomass Conversion and Biorefinery, 13(17): 16241-16251. http://dx.doi.org/10.1007/ s13399-023-04662-3 CR - Jayaprakash, G., Bains, A., Chawla, P., Fogarasi, M., Fogarasi, S. (2022). A narrative review on rice proteins: Current scenario and food industrial application. Polymers, 14(15): 3003. https://doi.org/10.3390/polym14153003 CR - Kim, W., Wang, Y., Selomulya, C. (2020). Dairy and plant proteins as natural food emulsifiers. Trends in Food Science & Technology, 105: 261-272. https://doi.org/10.1016/j.tifs.2020.09.012 CR - Khalesi, M., Glenn-Davi, K., Mohammadi, N., FitzGerald, R. J. (2024). Key factors ınfluencing gelation in plant vs. animal proteins: A comparative mini-review. Gels, 10(9): 575. https://doi.org/10.3390/gels10090575 CR - Kostić, A. Ž., Barać, M. B., Stanojević, S. P., Milojković-Opsenica, D. M., Tešić, Ž. L., Šikoparija, B., Pešić, M. B. (2015). Physicochemical composition and techno-functional properties of bee pollen collected in Serbia. LWT-Food Science and Technology, 62(1): 301-309. https://doi.org/10.1016/ j.lwt.2015.01.031 CR - Kyriakopoulou, K., Keppler, J. K., van Der Goot, A. J. (2021). Functionality of ingredients and additives in plant-based meat analogues. Foods, 10(3): 600. https://doi.org/10.3390/ foods10030600 CR - Lee, S. Y., Show, P. L., Ling, T. C., Chang, J. S. (2017). Single-step disruption and protein recovery from Chlorella vulgaris using ultrasonication and ionic liquid buffer aqueous solutions as extractive solvents. Biochemical Engineering Journal, 124: 26-35. https://doi.org/ 10.1016/j.bej.2017.04.009 CR - Liu, C., Damodaran, S., Heinonen, M. (2019). Effects of microbial transglutaminase treatment on physiochemical properties and emulsifying functionality of faba bean protein isolate. Lwt, 99: 396-403. https://doi.org/10.1016/ j.lwt.2018.10.003 CR - Liu, X., Wang, M., Xue, F., Adhikari, B. (2022). Application of ultrasound treatment to improve the technofunctional properties of hemp protein isolate. Future Foods, 6: 100176. https://doi.org/10.1016/j.fufo.2022.100176 CR - Liu, Y., Wu, Q., Zhang, J., Yan, W., Mao, X. (2024). Food emulsions stabilized by proteins and emulsifiers: A review of the mechanistic explorations. International Journal of Biological Macromolecules, 261(1): 129795. https://doi.org/ 10.1016/j.ijbiomac.2024.129795 CR - Loushigam, G., Shanmugam, A. (2023). Modifications to functional and biological properties of proteins of cowpea pulse crop by ultrasound-assisted extraction. Ultrasonics Sonochemistry, 97: 106448. https://doi.org/10.1016/j.ultsonch.2023.106448 CR - Luo, L., Cheng, L., Zhang, R., Yang, Z. (2022). Impact of high-pressure homogenization on physico-chemical, structural, and rheological properties of quinoa protein isolates. Food Structure, 32: 100265. https://doi.org/10.1016/j.foostr.2022.100265 CR - Loveday, S. M. (2020). Plant protein ingredients with food functionality potential. Nutrition Bulletin, 45(3): 321-327. https://doi.org/10.1111/nbu.12450 CR - Ma, Y., Chen, F. (2023). Plant protein heat-induced gels: Formation mechanisms and regulatory strategies. Coatings, 13(11): 1899. https://doi.org/10.3390/coatings13111899 CR - Małecki, J., Muszyński, S., Sołowiej, B. G. (2021). Proteins in food systems—bionanomaterials, conventional and unconventional sources, functional properties, and development opportunities. Polymers, 13(15): 2506. https://doi.org/10.3390/polym13152506 CR - Maribao, I. P., Gul, O. (2024). Effect of high-pressure homogenization pretreatment on gelation behavior and physicochemical, rheological and structural properties of sesame protein isolate with glucono-δ-lactone. Food Hydrocolloids, 155: 110240. https://doi.org/10.1016/j.foodhyd.2024.110240 CR - Mateo-Roque, P., Morales-Camacho, J. I., Jara-Romero, G. J., Rosas-Cárdenas, F. D. F., Huerta-González, L., Luna-Suárez, S. (2024). Supercritical CO2 treatment to modify techno-functional properties of proteins extracted from tomato seeds. Foods, 13(7): 1045. https://doi.org/10.3390/foods13071045 CR - Mathews, A., Tangirala, A. S., Kumar, S., Anandharaj, A., Rawson, A. (2023). Extraction and modification of protein from sesame oil cake by the application of emerging technologies. Food Chemistry Advances, 2: 100326. https://doi.org/10.1016/j.focha.2023.100326 CR - McClements, D. J., Bai, L., Chung, C. (2017). Recent advances in the utilization of natural emulsifiers to form and stabilize emulsions. Annual review of food science and technology, 8(1): 205-236. https://doi.org/10.1146/annurev-food-030216-030154 CR - McClements, D. J., Grossmann, L. (2021). The science of plant‐based foods: Constructing next‐generation meat, fish, milk, and egg analogs. Comprehensive Reviews in Food Science and Food Safety, 20(4): 4049-4100. https://doi.org/10.1111/1541-4337.12771 CR - Naliyadhara, N., Kumar, A., Girisa, S., Daimary, U. D., Hegde, M., Kunnumakkara, A. B. (2022). Pulsed electric field (PEF): Avant-garde extraction escalation technology in food industry. Trends in Food Science & Technology, 122: 238-255. https://doi.org/10.1016/j.tifs.2022.02.019 CR - Nasrabadi, M. N., Doost, A. S., Mezzenga, R. (2021). Modification approaches of plant-based proteins to improve their techno-functionality and use in food products. Food Hydrocolloids, 118: 106789. https://doi.org/10.1016/ j.foodhyd.2021.106789 CR - Nasrollahzadeh, M., Nezafat, Z., Shafiei, N. (2021). Proteins in food industry. Biopolym.-Based Met. Nanopart. Chem. Sustain. Appl, 2: 97-136. http://dx.doi.org/10.1016/B978-0-323-89970-3.00003-2 CR - Ngo, N. T. T., Shahidi, F. (2021). Functional properties of protein isolates from camelina (Camelina sativa (L.) Crantz) and flixweed (sophia, Descurainis sophia L.) seed meals. Food Production, Processing and Nutrition, 3: 1-10. http://dx.doi.org/10.1186/s43014-021-00076-8 CR - Ochoa-Rivas, A., Nava-Valdez, Y., Serna-Saldívar, S. O., Chuck-Hernández, C. (2017). Microwave and ultrasound to enhance protein extraction from peanut flour under alkaline conditions: Effects in yield and functional properties of protein isolates. Food and Bioprocess Technology, 10: 543-555. https://dx.doi.org/ 10.1007/s11947-016-1838-3 CR - Ortega, M. L. S., Orellana-Palacios, J. C., Garcia, S. R., Rabanal-Ruiz, Y., Moreno, A., Hadidi, M. (2024). Olive leaf protein: Extraction optimization, in vitro digestibility, structural and techno-functional properties. International Journal of Biological Macromolecules, 256: 128273. https://doi.org/10.1016/j.ijbiomac.2023.128273 CR - Ozkan, G., Tataroglu, P., Gulec, S., Capanoglu, E. (2024). Modification of pea protein isolates by high-intensity ultrasonication: Functional, structural and nutritional properties. Food Chemistry Advances, 5: 100793. https://doi.org/ 10.1016/j.focha.2024.100793 CR - Parlak, M. E., Sarıcaoglu, F. T., Yilmaz, M. T. (2024). Application of high-pressure homogenization-assisted pH-shift to enhance techno-functional and interfacial properties of lentil protein isolate. Food Hydrocolloids, 157: 110425. https://doi.org/10.1016/ j.foodhyd.2024.110425 CR - Phongthai, S., Lim, S. T., Rawdkuen, S. (2016). Optimization of microwave-assisted extraction of rice bran protein and its hydrolysates properties. Journal of cereal science, 70: 146-154. https://doi.org/10.1016/j.jcs.2016.06.001 CR - Ptak-Kaczor, M., Banach, M., Stapor, K., Fabian, P., Konieczny, L., Roterman, I. (2021). Solubility and aggregation of selected proteins interpreted on the basis of hydrophobicity distribution. International Journal of Molecular Sciences, 22(9): 5002. https://doi.org/10.3390/ijms22095002 CR - Ricardo, F., Pradilla, D., Cruz, J. C., Alvarez, O. (2021). Emerging emulsifiers: Conceptual basis for the identification and rational design of peptides with surface activity. International Journal of Molecular Sciences, 22(9): 4615. https://doi.org/10.3390/ijms22094615 CR - Rudke, C. R. M., Torres, T. M. S., Zielinski, A. A. F., Ferreira, S. R. S. (2024). Comparing green extraction methods for the recovery of protein-rich fraction from peach seeds (Prunus persica). Food Hydrocolloids, 153: 109991. https://doi.org/10.1016/j.tifs.2020.01.011 CR - Sá, A. G. A., Moreno, Y. M. F., Carciofi, B. A. M. (2020). Plant proteins as high-quality nutritional source for human diet. Trends in Food Science & Technology, 97: 170-184. https://doi.org/10.1016/ j.tifs.2020.01.011 CR - Santos, F. H., de Carvalho Oliveira, L., de Sousa Melo, D., Bakalis, S., Cristianini, M. (2024). Modification of protein concentrate from carioca bean (Phaseolus vulgaris L.) by dynamic high-pressure technology: Structural and techno-functional properties. Innovative Food Science & Emerging Technologies, 97: 103823. https://doi.org/10.1016/j.ifset.2024.103823 CR - Sathe, S. K., Zaffran, V. D., Gupta, S., Li, T. (2018). Protein solubilization. Journal of the American Oil Chemists' Society, 95(8): 883-901. https://doi.org/10.1002/aocs.12058 CR - Saricaoglu, F. T. (2020). Application of high-pressure homogenization (HPH) to modify functional, structural and rheological properties of lentil (Lens culinaris) proteins. International Journal of Biological Macromolecules, 144: 760-769. https://doi.org/10.1016/j.ijbiomac.2019.11.034 CR - Savarino, G., Corsello, A., Corsello, G. (2021). Macronutrient balance and micronutrient amounts through growth and development. Italian journal of pediatrics, 47(1): 109. https://doi.org/10.1186/s13052-021-01061-0 CR - Shah, H., Ahmed, L., Barry-Ryan, C. (2024). Ensuring compliance: A review of EU regulations and standards for incorporating legume and legume by-product proteins in food formulations. Heliyon, 10(21): e39821. http://dx.doi.org/ 10.1016/j.heliyon.2024.e39821 CR - Shevkani, K., Sharma, P., Singh, B., Kaur, A., Singh, N. (2024). Structural, techno‐functional and nutraceutical properties of lentil proteins–a concise review. International Journal of Food Science & Technology, 59(10): 6875-6884. http://dx.doi.org/10.1111/ijfs.17491 CR - Shinwari, K. J. (2021). Emerging technologies for the recovery of bioactive compounds from saffron species. Saffron, 143-182. https://doi.org/10.1016/B978-0-12-821219-6.00004-X CR - Sim, S. Y. J., Srv, A., Chiang, J. H., Henry, C. J. (2021). Plant proteins for future foods: A roadmap. Foods, 10(8): 1967. https://doi.org/ 10.3390/foods10081967 CR - Srinivasu, S. R., Eligar, S. M. (2024). Physico-chemical and techno-functional characterization of quinoa bran protein concentrate. Journal of Cereal Science, 116: 103835. http://dx.doi.org/ 10.1016/j.jcs.2023.103835 CR - Suárez-Hernández, M. F., Posada Ramirez, S. G., Castillo Cruz, D. D. C., Higuera Ciapara, I., Pacheco López, N. A., Herrera Pool, I. E., Ruiz-Ruiz, J. C. (2024). Effect of ıntegrated extraction techniques on the technofunctional and bioactive properties of Brosimum alicastrum Swartz proteins. Foods, 13(18): 2875. https://doi.org/ 10.3390/foods13182875 CR - Tang, J., Yao, D., Xia, S., Cheong, L. Z., Tu, M. (2024). Recent progress in plant-based proteins: From extraction and modification methods to applications in the food industry. Food Chemistry: X, 23: 101540. https://doi.org/10.1016/ j.fochx.2024.101540 CR - Thongkong, S., Klangpetch, W., Unban, K., Tangjaidee, P., Phimolsiripol, Y., Rachtanapun, P., Phongthai, S. (2023). Impacts of electroextraction using the pulsed electric field on properties of Rice bran protein. Foods, 12(4): 835. https://doi.org/10.3390/foods12040835 CR - Varghese, T., Pare, A. (2019). Effect of microwave assisted extraction on yield and protein characteristics of soymilk. Journal of Food Engineering, 262: 92-99. https://doi.org/10.1016/ j.jfoodeng.2019.05.020 CR - Vogelsang-O’Dwyer, M., Sahin, A. W., Arendt, E. K., Zannini, E. (2022). Enzymatic hydrolysis of pulse proteins as a tool to improve techno-functional properties. Foods, 11(9): 1307. https://doi.org/10.3390/foods11091307 CR - Yusoff, I. M., Taher, Z. M., Rahmat, Z., Chua, L. S. (2022). A review of ultrasound-assisted extraction for plant bioactive compounds: Phenolics, flavonoids, thymols, saponins and proteins. Food research international, 157: 111268. https://doi.org/10.1016/j.foodres.2022.111268 CR - Zhang, L., Wang, L. J., Jiang, W., Qian, J. Y. (2017). Effect of pulsed electric field on functional and structural properties of canola protein by pretreating seeds to elevate oil yield. Lwt, 84: 73-81. https://doi.org/10.1016/ j.lwt.2017.05.048 CR - Zhang, X., Wang, Q., Liu, Z., Zhi, L., Jiao, B., Hu, H., Shi, A. (2023). Plant protein-based emulsifiers: Mechanisms, techniques for emulsification enhancement and applications. Food Hydrocolloids, 144: 109008. https://doi.org/10.1016/ j.foodhyd.2023.109008 CR - Wang, Q., Wang, Y., Huang, M., Hayat, K., Kurtz, N. C., Wu, X., Zheng, F. (2021). Ultrasound-assisted alkaline proteinase extraction enhances the yield of pecan protein and modifies its functional properties. Ultrasonics Sonochemistry, 80: 105789. https://doi.org/10.1016/ j.ultsonch.2021.105789 UR - https://doi.org/10.15237/10.15237/gida.GD25026 L1 - https://dergipark.org.tr/tr/download/article-file/4595677 ER -