TY  - JOUR
T1  - Cytotoxic and Metastatic Effects of Anatolian Propolis and Chemotherapeutic Agents (DOX, TAM, CLB) in 2D and 3D Breast Cancer Models
TT  - Anadolu Propolisi ve Kemoterapötik Ajanların (DOX, TAM, CLB) 2B ve 3B Meme Kanseri Modellerindeki Sitotoksik ve Metastatik Etkileri
AU  - Arslan, Habibe Sema
AU  - Yalçın Azarkan, Serap
AU  - Turna Saltoğlu, Gamze
PY  - 2025
DA  - June
Y2  - 2025
DO  - 10.35206/jan.1639903
JF  - Journal of Apitherapy and Nature
JO  - J.Apit.Nat.
PB  - Oktay YILDIZ
WT  - DergiPark
SN  - 2667-4734
SP  - 103
EP  - 127
VL  - 8
IS  - 1
LA  - en
AB  - This study aims to comparatively investigate the cytotoxic and metastatic effects of Anatolian propolis and chemotherapeutic agents (Doxorubicin- DOX, Tamoxifen- TAM, Chlorambucil- CLB) on two-dimensional (2D) and three-dimensional (3D) breast cancer cell cultures. The triple-negative MDA-MB-231 breast cancer cell line was cultured in 2D and 3D models. Anatolian propolis was prepared using ethanol extraction and applied to the cells alone or in combination with chemotherapeutic agents (DOX, TAM, CLB) at their respective IC50 doses. Cytotoxicity was assessed using the XTT assay, while cell migration and invasion were evaluated using wound healing and invasion assays. Propolis, when combined with chemotherapeutic agents, significantly inhibited cell proliferation and migration (p
KW  - Propolis
KW  - Doxorubicin
KW  - Tamoxifen
KW  - Chlorambucil
KW  - MDA-MB-231
N2  - Bu çalışma, Anadolu propolisi ve kemoterapötik ajanların (Doksorubisin- DOX, Tamoksifen- TAM, Klorambusil- CLB) iki boyutlu (2B) ve üç boyutlu (3B) meme kanseri hücre kültürleri üzerindeki sitotoksik ve metastatik etkilerini karşılaştırmalı olarak incelemeyi amaçlamaktadır. Çalışmada, MDA-MB-231 üçlü negatif meme kanseri hücre hattı, 2B ve 3B kültür modellerinde geliştirilmiştir. Anadolu propolisi, etanol ekstraksiyonu yöntemiyle hazırlanmış ve belirlenen IC50 dozlarında kemoterapötik ajanlar (DOX, TAM, CLB) ile ayrı ayrı ve kombinasyon halinde hücrelere uygulanmıştır. Sitotoksisite XTT testi, hücre göçü ve invazyonu ise yara iyileştirme ve invazyon testleri ile değerlendirilmiştir. Propolis, kemoterapötik ajanlarla kombinasyon halinde uygulandığında, hücre proliferasyonunu ve migrasyonunu anlamlı şekilde baskılamıştır (p
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UR  - https://doi.org/10.35206/jan.1639903
L1  - https://dergipark.org.tr/tr/download/article-file/4609773
ER  -