@article{article_1651242, title={The accurate and reliable HPLC-UV based method for detecting the active ingredient hexaflumuron in some plant protection products.}, journal={Turkish Journal of Analytical Chemistry}, volume={7}, pages={162–167}, year={2025}, DOI={10.51435/turkjac.1651242}, author={Mohamed, Ismael}, keywords={Hexaflumuron, HPLC Analizi, Yöntem doğrulama, Hassasiyet, Doğruluk}, abstract={The main objective of this study was to present a new selective, sensitive, and validated RP-HPLC analytical method for measuring the concentrations of hexaflumuron (HFM) which is a well-known active ingredients that is widely used as an insecticide to protect crops, especially fruits and vegetables. Quantification was carried out using a reversed phase HPLC system that was equipped with a UV detector. The development of the novel method was performed on reversed-phase C-18 (stainless steel, 5 µm, 250 × 4.6 mm) column at constant temperature 30 ºC. The mobile phase consists of acetonitrile and distilled water in volumetric ratio 85:15, a flow rate of 1 mL/min, and the detection wavelength at 220 nm. Retention time of separation at 3.84 min. The method was validated by testing specificity, linearity, precision, recovery, LOD, LOQ, and accuracy according to the CIPAC (Collaborative International Pesticides Analytical Council) and comply with the guidelines of SANCO/3030/99 rev.5. guidelines. The method revealed an acceptable linearity regression R2 (0.9974). The method was found to be accurate from concentration levels 50–250 µg/mL with high accuracy (99.3-100.7%). The method’s validation results make it suitable for use as a standardization tool in the evaluation of Emulsion Concentrate formulations containing this active ingredient.}, number={2}, publisher={Miraç OCAK}, organization={KZ for Pesticides and Chemicals Company}