TY - JOUR T1 - CHARACTERIZATION OF GREEN SYNTHESIZED SILVER AND ZINC NANOPARTICLES USING PROPOLIS EXTRACT AND THEIR ANTIMICROBIAL, ANTIBIOFILM, ANTIQUORUM SENSING ACTIVITIES TT - PROPOLİS EKSTRESİ KULLANARAK YEŞİL SENTEZLENMİŞ GÜMÜŞ VE ÇİNKO NANOPARTİKÜLLERİNİN KARAKTERİZASYONU VE ANTİMİKROBİYAL, ANTİBİYOFİLM, ÇOĞUNLUĞU ALGILAMA İNHİBİSYONU AKTİVİTELERİ AU - Palabıyık, İsmail Murat AU - Vural, Neslinaz AU - Gökdere, Nuran AU - Rızvanoğlu, Suna Sibel AU - Bay, Mehmet AU - Biran Ay, Suzan AU - Eryılmaz, Müjde AU - Kosku Perkgöz, Nihan PY - 2025 DA - September Y2 - 2025 DO - 10.33483/jfpau.1657517 JF - Journal of Faculty of Pharmacy of Ankara University JO - J. Fac. Pharm. Ankara PB - Ankara Üniversitesi WT - DergiPark SN - 1015-3918 SP - 817 EP - 826 VL - 49 IS - 3 LA - en AB - Objective: AgNP and ZnO NP were synthesized by green synthesis method using propolis, the characterization of nanoparticles was examined and their antimicrobial, antibiofilm and antiquarum sensing activities were examined. Material and Method: Nanoparticles were synthesized from the prepared propolis extract using 100 ml and 25 ml of extract for AgNP and ZnONP, respectively. Characterization was performed using UV-Visible (UV-VIS) spectra, XRD, EDX and SEM. Antimicrobial, antibiofilm and anti-quorum sensing activities of the nanoparticles were examined. Result and Discussion: Looking at the characterization results, AgNP and ZnO NP with very small particle sizes were synthesized. According to the antibacterial activity results, the MIC values of ZnO NPs and AgNPs were determined as 0.0625 mg/ml and 0.0250 mg/ml against S. aureus ATCC 43300 (MRSA), respectively. The percentage biofilm inhibition value of ZnO NPs and AgNPs was found to be 38.94% and 38.67%, respectively. The synthesized AgNP and ZnO NPs did not show anti-quorum sensing activity. KW - Antibiofilm KW - antimicrobial KW - antiquorum sensing KW - characterization KW - green synthesis KW - silver nanoparticle KW - zinc nanoparticle N2 - Amaç: Propolis kullanılarak yeşil sentez yöntemi ile AgNP ve ZnO NP sentezlendi, nanopartiküllerin karakterizasyonu incelendi ve antimikrobiyal, antibiyofilm ve çoğunluğu algılama inhibisyonu aktiviteleri incelendi.Gereç ve Yöntem: Hazırlanan propolis ekstresinden AgNP ve ZnONP için sırasıyla 100 ml ve 25 ml ekstre kullanılarak nanopartiküller sentezlendi. Karakterizasyon, UV-Görünür bölge (UV-VIS) spektroskopisi, XRD, EDX ve SEM kullanılarak gerçekleştirildi. Nanopartiküllerin antimikrobiyal, antibiyofilm ve çoğunluğu algılama inhibisyonu aktiviteleri incelendi.Sonuç ve Tartışma: Karakterizasyon sonuçlarına bakıldığında oldukça küçük partikül boyutuna sahip AgNP ve ZnO NP sentezlendi. Antibakteriyel aktivite sonuçlarına göre ZnO NP'leri ve AgNP'leri S. aureus ATCC 43300 (MRSA)'ya karşı sırasıyla 0.0625 mg/ml ve 0.0250 mg/ml MİK değerleri gösterdi. ZnO NP'leri ve AgNP'lerinin biyofilm inhibisyon yüzdesi değeri sırasıyla %38.94 ve %38.67 olarak bulundu. Sentezlenen AgNP ve ZnO NP çoğunluğu algılama inhibisyonu aktivitesi göstermedi. CR - 1. Sforcin, J.M. (2007). Propolis and the immune system: A review. Journal of Ethnopharmacology, 113(1), 1-14. [CrossRef] CR - 2. Bankova, V.S., de Castro, S.L., Marcucci, M.C. (2000). Propolis: Recent advances in chemistry and plant origin. Apidologie, 31(1), 3-15. [CrossRef] CR - 3. Hames-Kocabas, E.E., Demirci, B., Uzel, A., Demirci, F. (2013). Volatile composition of Anatolian propolis by headspace-solid-phase microextraction (HS-SPME), antimicrobial activity against food contaminants and antioxidant activity. Journal of Medicinal Plants Research, 7(28), 2140-2149. [CrossRef] CR - 4. Toreti, V.C., Sato, H.H., Pastore, G.M., Park, Y.K. (2013). Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence‐Based Complementary and Alternative Medicine, 2013(1), 697390. [CrossRef] CR - 5. Xu, Y., Luo, L., Chen, B., Fu, Y. (2009). Recent development of chemical components in propolis. Frontiers of Biology in China, 4, 385-391. [CrossRef] CR - 6. Tatli Seven, P., Seven, I., Gul Baykalir, B., Iflazoglu Mutlu, S., Salem, A.Z. (2018). Nanotechnology and nano-propolis in animal production and health: An overview. Italian Journal of Animal Science, 17(4), 921-930. [CrossRef] CR - 7. Yadi, M., Mostafavi, E., Saleh, B., Davaran, S., Aliyeva, I., Khalilov, R., Nikzamir, M., Nikzamir, N., Akbarzadeh, A., Panahi, Y., Milani, M. (2018). Current developments in green synthesis of metallic nanoparticles using plant extracts: A review. Artificial Cells, Nanomedicine, and Biotechnology, 46(sup3), 336-343. [CrossRef] CR - 8. Priyadarshini, J.F., Sivakumari, K., Selvaraj, R., Ashok, K., Jayaprakash, P., Rajesh, S. (2018). Green synthesis of silver nanoparticles from propolis. Research Journal of Life Sciences, Bioinformatics, Pharmaceutical and Chemical Sciences, 4, 23-36. CR - 9. Taqi, Z.J., Abdul-Wahed, H.E., AL-Saadi, H.K., Jabir, M. (2020). Potential activity of silver nanoparticles synthesized by Iraqi propolis on phagocytosis. In AIP Conference Proceedings, 2213(1), 020104. [CrossRef] CR - 10. Dubey, S.P., Lahtinen, M., Sillanpää, M. (2010). Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugosa. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 364(1-3), 34-41. [CrossRef] CR - 11. Barbosa, V.T., Souza, J.K., Alvino, V., Meneghetti, M.R., Florez‐Rodriguez, P.P., Moreira, R.E., Paulino, G.V.B., Landell, M.F., Basílio-Júnior, I.D., Nascimento, T.G., Grillo, L.A.M., Dornelas, C.B. (2019). Biogenic synthesis of silver nanoparticles using Brazilian propolis. Biotechnology Progress, 35(6), e2888. [CrossRef] CR - 12. Barsola, B., Kumari, P. (2022). Green synthesis of nano-propolis and nanoparticles (Se and Ag) from ethanolic extract of propolis, their biochemical characterization: A review. Green Processing and Synthesis, 11(1), 659-673. [CrossRef] CR - 13. Ventola, C.L. (2015). The antibiotic resistance crisis: Part 1: Causes and threats. Pharmacy and Therapeutics, 40(4), 277. CR - 14. Brown, E.D., Wright, G.D. (2016). Antibacterial drug discovery in the resistance era. Nature, 529(7586), 336-343. [CrossRef] CR - 15. Hall-Stoodley, L., Costerton, J.W., Stoodley, P. (2004). Bacterial biofilms: From the natural environment to infectious diseases. Nature Reviews Microbiology, 2(2), 95-108. [CrossRef] CR - 16. Miller, M. B., Bassler, B.L. (2001). Quorum sensing in bacteria. Annual Review of Microbiology, 55, 165-199. [CrossRef] CR - 17. Rai, M., Yadav, A., Gade, A. (2021). Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 27(1), 76-83. [CrossRef] CR - 18. Junejo, B., Eryilmaz, M., Rizvanoglu, S.S., Palabiyik, I.M., Ghumro, T., Mallah, A., Solangi, A.R., Hyder, S.I., Maleh, K.H., Dragoi, E.N. (2023). Pharmacological assessment of Co3O4, CuO, NiO and ZnO nanoparticles via antibacterial, anti-biofilm and anti-quorum sensing activities. Water Science & Technology, 87(11), 2840-2851. [CrossRef] CR - 19. Sahlan, M., Supardi, T. (2013). Encapsulation of Indonesian propolis by casein micelle. International Journal of Pharma and Bio Sciences, 4(1), 297-305. CR - 20. Dashtizadeh, Z., Kashi, F.J., Ashrafi, M. (2021). Phytosynthesis of copper nanoparticles using Prunus mahaleb L. and its biological activity. Materials Today Communications, 27, 102456. [CrossRef] CR - 21. Clinical and Laboratory Standards Institute (CLSI) Web site (2024). M07-A8 methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard, Nineteenth informational supplement, USA. Retrieved January, 2009, from https://clsi.org/. Accessed date: 03.04.2024. CR - 22. Clinical and Laboratory Standards Institute (CLSI) Web site (2023). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard, Fourth informational supplement, USA. Retrieved November, 2017, from https://clsi.org/. Accessed date: 08.06.2023. CR - 23. Paulkumar, K., Gnanajobitha, G., Vanaja, M., Rajeshkumar, S., Malarkodi, C., Pandian, K., Annadurai, G. (2014). Piper nigrum leaf and stem assisted green synthesis of silver nanoparticles and evaluation of its antibacterial activity against agricultural plant pathogens. The Scientific World Journal, 2014(1), 829894. [CrossRef] CR - 24. Nagaraju, G., Prashanth, S.A., Shastri, M., Yathish, K.V., Anupama, C., Rangappa, D.J.M.R.B. (2017). Electrochemical heavy metal detection, photocatalytic, photoluminescence, biodiesel production and antibacterial activities of Ag–ZnO nanomaterial. Materials Research Bulletin, 94, 54-63. [CrossRef] UR - https://doi.org/10.33483/jfpau.1657517 L1 - https://dergipark.org.tr/tr/download/article-file/4689309 ER -