@article{article_1663961, title={INTERACTION BETWEEN ELTROMBOPAG AND HUMAN SERUM ALBUMIN: FLUORESCENCE AND ELECTROCHEMICAL STUDIES}, journal={Journal of Faculty of Pharmacy of Ankara University}, volume={49}, pages={859–869}, year={2025}, DOI={10.33483/jfpau.1663961}, author={Yence, Merve and Çetinkaya, Ahmet and Bellur Atici, Esen and Özkan, Sibel A.}, keywords={Diferansiyel darbe voltametrisi, eltrombopag, floresan spektroskopisi, ilaç protein etkileşimi, insan serum albümini}, abstract={Objective: This research investigated the interaction between Eltrombopag (EB) and Human Serum Albumin (HSA) by fluorescence spectroscopy and voltammetry while explaining the quenching mechanism.
Material and Method: The electrochemical studies were conducted in an acetate buffer solution (AB) at pH 4 by differential pulse voltammetry (DPV). For the fluorescence studies, EB, HSA, and a mixture of EB and HSA solutions were designed with a pH of 4.7, AB containing 20% DMSO.
Result and Discussion: The interaction between EB and HSA was examined by fluorescence and electrochemical titrations, which showed a quenching effect and peak shifting. Fluorescence titrations indicate that EB’s HSA quenching process is static and has a hypsochromic shift. Analysis of thermodynamic parameters and higher binding constants concluded a strong and spontaneous interaction. Electrochemical titrations show the intercalation of EB into HSA.}, number={3}, publisher={Ankara Üniversitesi}