TY  - JOUR
T1  - Determination of Antioxidant, Cytotoxic, Apoptotic/Necrotic and Gene Expression Levels of Calculus bovis Extract
TT  - Calculus bovis Ekstraktının Antioksidan, Sitotoksik, Apoptotik/Nekrotik ve Gen Ekspresyon Düzeylerinin Belirlenmesi
AU  - Çil, Ayşenur
AU  - Dalkılıç, Semih
AU  - Kadıoğlu Dalkılıç, Lütfiye
AU  - Akay, Gökhan
AU  - Korkmaz, İsmail
AU  - Fidan, Songül
PY  - 2025
DA  - October
Y2  - 2025
DO  - 10.55024/buyasambid.1665035
JF  - Batman Üniversitesi Yaşam Bilimleri Dergisi
JO  - Batman univ. yaşam bilim. derg.
PB  - Batman Üniversitesi
WT  - DergiPark
SN  - 2147-4877
SP  - 1
EP  - 20
VL  - 15
IS  - 1
LA  - en
AB  - Cancer kills millions of people every year and affects all of humanity. 22 million new cases are projected by 2030. Side effects of standard chemotherapeutic agents and tumor recurrence after treatment have led to the development of molecules derived from natural and edible products. In this study, the impact of Calculus bovis extracts on cancer cells was examined. In MDA-MB-231, A549, PANC-1, and OVCAR-3 cell lines, cytotoxic activity was evaluated by MTT assay. The apoptotic/necrotic effect was analyzed by the double staining method. Bax, Bcl-2, p21, and p53 gene expression levels were measured by qRT-PCR in methanol extract in the OVCAR-3 cell line. The results showed that acetone extract showed a significant cytotoxic effect in the OVCAR-3 cell line. In the same cell line, the methanol extract suppressed Bcl-2 gene expression and triggered apoptosis. The findings suggest that Calculus bovis may be a potential chemotherapeutic agent, especially for the treatment of ovarian and breast cancer. We expect the extract to be effective against different cancer cell lines without side effects. However, its side effect profile is yet to be determined and needs further research.
KW  - Cytotoxicity
KW  - Apoptosis
KW  - Necrosis
KW  - Antioxidant
KW  - Gene expression
N2  - Kanser, her yıl milyonlarca insanın ölümüne neden olmakta ve tüm insanlığı etkilemektedir. 2030 yılına kadar 22 milyon yeni vaka öngörülmektedir. Standart kemoterapötik ajanların yan etkileri ve tedavi sonrası tümör tekrarı, doğal ve yenilebilir ürünlerden elde edilen moleküllerin geliştirilmesine yönlendirmiştir. Bu çalışmada, Calculus bovis ekstraktlarının kanser hücreleri üzerindeki etkileri incelenmiştir. MDA-MB-231, A549, PANC-1 ve OVCAR-3 hücre hatlarında MTT testi ile sitotoksik aktivite değerlendirilmiştir. Apoptotik/nekrotik etki çift boyama yöntemi ile analiz edilmiştir. Bax, Bcl-2, p21 ve p53 gen ekspresyon seviyeleri OVCAR-3 hücre hattında metanol ektraktında qRT-PCR yöntemiyle ölçülmüştür. Elde edilen sonuçlar gösteriyor ki aseton ekstraktı, OVCAR-3 hücre hattında belirgin sitotoksik etki göstermiştir. Aynı hücre hattında metanol ektraktında Bcl-2 gen ekspresyonunun baskılandığı ve apoptozu tetiklediği belirlenmiştir. Bulgular, Calculus bovis’in özellikle over ve meme kanseri tedavisi için potansiyel bir kemoterapötik ajan olabileceğini göstermektedir. Ekstraktın farklı kanser hücre hatlarına karşı yan etki göstermeden etkili olacağını umuyoruz. Ancak, yan etki profili henüz belirlenmediğinden araştırması gerekmektedir.
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UR  - https://doi.org/10.55024/buyasambid.1665035
L1  - https://dergipark.org.tr/tr/download/article-file/4723063
ER  -