TY  - JOUR
T1  - Kars ili Endemik ve Nadir Bitki Türlerinin Tanımlanmasında ITS1 ve ITS2 Gen Bölgelerinin Etkinliği
TT  - The Effectiveness of ITS1 and ITS2 Gene Regions in the Identification of Endemic and Rare Plant Species in Kars Province
AU  - Özdemir Değirmenci, Funda
AU  - Uluğ, Asiye
AU  - Akdoğan Karadağ, Gül Esma
AU  - Özgökçe, Fevzi
PY  - 2025
DA  - August
Y2  - 2025
DO  - 10.53433/yyufbed.1670379
JF  - Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi
JO  - YYUFBED
PB  - Van Yüzüncü Yıl Üniversitesi
WT  - DergiPark
SN  - 1300-5413
SP  - 485
EP  - 498
VL  - 30
IS  - 2
LA  - tr
AB  - DNA barkodlama, bitki türlerinin genetik düzeyde doğru ve güvenilir şekilde tanımlanmasını sağlayan etkili bir yöntemdir. Bu çalışmada, Kars iline özgü endemik bitki türlerinin moleküler karakterizasyonu ve filogenetik ilişkilerinin belirlenmesi amacıyla ITS1 ve ITS2 barkod gen bölgeleri kullanılmıştır. Elde edilen DNA dizileri, her iki bölgenin de moleküler tanımlama için yüksek çözünürlük sunduğunu, ancak ITS2 bölgesinin tür düzeyinde daha fazla bilgi içeriği ve yüksek nükleotit çeşitliliği sağladığını göstermektedir.  Genetik çeşitlilik analizleri sonucunda bazı türlerde yüksek nükleotit çeşitliliği ve özgün mutasyonlara rastlanırken, bazı türlerde ise oldukça korunmuş dizilere ve sınırlı düzeyde genetik farklılıklara rastlanmıştır. ITS1 ve ITS2 bölgelerinin birlikte kullanımı, taksonomik sınıflandırmanın daha güvenilir ve kapsamlı bir şekilde gerçekleştirilmesine olanak tanımaktadır. Bu çalışmada, iki gen bölgesi için elde edilen ve veri tabanına yüklenen genetik barkodlar, Türkiye’nin, özellikle Kars ilinin biyolojik çeşitliliğinin ulusal ve uluslararası düzeyde belgelenmesine ve korunmasına önemli bir katkı sunacaktır. Ayrıca, çalışma sonuçları bölgenin biyocoğrafyası ve ekolojik süreçlerine ilişkin yeni araştırmalara bilimsel bir temel sağlayarak, biyolojik çeşitliliğin korunması ve taksonomik doğrulama çalışmalarına değerli bir kaynak oluşturma potansiyeline sahiptir.
KW  - DNA barkodlama
KW  - Endemik
KW  - Filogenetik
KW  - ITS1
KW  - ITS2
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UR  - https://doi.org/10.53433/yyufbed.1670379
L1  - https://dergipark.org.tr/tr/download/article-file/4746906
ER  -