@article{article_1677781, title={Spermatological and Biochemical Examination of New Zealand Rabbit Semen After Thawing}, journal={Kocatepe Veterinary Journal}, volume={18}, pages={207–214}, year={2025}, DOI={10.30607/kvj.1677781}, author={Ozturk, Caner and Döşeyici, Mustafa Eren and Aksoy, Neşe Hayat}, keywords={kafeik asit, kriyoprezervasyon, floresan boyama, oksidatif stres, tavşan sperması}, abstract={In the present study, the effects of caffeic acid on rabbit semen were investigated using the antioxidant substance trehalose as a positive control. In the experiment, 4 male rabbits and 1 female rabbit were used. Ejaculates were collected via the artificial vaginal method 2 times a week for 4 weeks. Pooled semen samples were divided into four groups and diluted as follows: basic diluent+6%DMSO+caffeic acid (25µM and 50µM), basic diluent+6%DMSO+trehalose (positive control) (50mM), and basic diluent+6%DMSO (Control). After dilution, the semen samples were aspirated into straws and allowed to equilibrate at +4°C for 1 h. The samples were frozen in liquid nitrogen vapor and stored in liquid nitrogen. The highest value of sperm motility was detected in the 50 mM trehalose group (46.25±1.25%), which was significantly different from that in the control group (35.63±1.99%) (p<0.05). The sperm membrane integrity (SYBR 14/PI) results were determined to be 47.11±0.96%, 49.05±0.85%, and 49.79±1.04% in the 25 µM caffeic acid, 50µM caffeic acid, and 50 mM trehalose groups, respectively, and a significant difference was found between the supplement groups and the control group (39.01±1.21%) (p<0.05). In terms of acrosome integrity (FITC-PNA), the highest value was reached in the trehalose 50 mM group (48.70±1.03%), and a significant difference was observed compared with that in the control group (41.48±0.80%). A significant difference was observed in the total antioxidant capacity and total oxidant level values between the 50 mM trehalose group and the control group (p<0.05). As a result, caffeic acid added to the extender when rabbit semen was frozen had a better protective effect at higher doses.}, number={3}, publisher={Afyon Kocatepe Üniversitesi}, organization={This research was funded by The Scientific and Technological Research Council of Turkey (TÜBİTAK) with number 1919B011802699}