TY  - JOUR
T1  - Synergistic Apoptotic Effects of Cisplatin and Arbutin in MCF-7 Breast Cancer Cells
TT  - Synergistic Apoptotic Effects of Cisplatin and Arbutin in MCF-7 Breast Cancer Cells
AU  - Kılıç, Mahmut Alp
AU  - Bozkurt Girit, Özlem
AU  - Kolaç, Umut Kerem
AU  - Goker Bagca, Bakiye
PY  - 2025
DA  - May
Y2  - 2025
DO  - 10.30934/kusbed.1678429
JF  - Kocaeli Üniversitesi Sağlık Bilimleri Dergisi
JO  - KOU Sag Bil Derg
PB  - Kocaeli Üniversitesi
WT  - DergiPark
SN  - 2149-8571
SP  - 70
EP  - 77
VL  - 11
IS  - 2
LA  - en
AB  - Objective: Cisplatin is a key component in cancer treatment, but its effectiveness can be limited by dose related toxicities. Combining it with natural compounds such as arbutin offers a promising approach to improve treatment outcomes while reducing side effects. This study aimed to explore the combined apoptotic effects of arbutin and cisplatin in MCF-7 breast cancer cells, specifically focusing on mitochondrial gene expression.Methods: MCF-7 cells were treated for 48 hours with arbutin, cisplatin, or a combination of both at fixed ratios. Cytotoxicity and synergy were evaluated using the Chou-Talalay median-effect method. Nuclear morphology, indicative of apoptosis, was assessed through Hoechst 33342 staining. Gene expression analysis targeted mitochondrial dynamics (DRP1, Fis1, MFN1, MFN2), oxidative stress markers (SOD2, GPx), apoptosis indicators (Bcl2), autophagy (Beclin1), and prostaglandin pathways (PGF2α, PGF2β), with results normalized to β-actin.Results: The combination therapy significantly enhanced cytotoxicity compared to individual treatments (Combination Index
KW  - Cisplatin
KW  - Arbutin
KW  - MCF-7
KW  - Apoptosis
KW  - Mitochondria
N2  - Objective: Cisplatin is a key component in cancer treatment, but its effectiveness can be limited by dose related toxicities. Combining it with natural compounds such as arbutin offers a promising approach to improve treatment outcomes while reducing side effects. This study aimed to explore the combined apoptotic effects of arbutin and cisplatin in MCF-7 breast cancer cells, specifically focusing on mitochondrial gene expression.Methods: MCF-7 cells were treated for 48 hours with arbutin, cisplatin, or a combination of both at fixed ratios. Cytotoxicity and synergy were evaluated using the Chou-Talalay median-effect method. Nuclear morphology, indicative of apoptosis, was assessed through Hoechst 33342 staining. Gene expression analysis targeted mitochondrial dynamics (DRP1, Fis1, MFN1, MFN2), oxidative stress markers (SOD2, GPx), apoptosis indicators (Bcl2), autophagy (Beclin1), and prostaglandin pathways (PGF2α, PGF2β), with results normalized to β-actin.Results: The combination therapy significantly enhanced cytotoxicity compared to individual treatments (Combination Index
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UR  - https://doi.org/10.30934/kusbed.1678429
L1  - https://dergipark.org.tr/tr/download/article-file/4783298
ER  -