TY - JOUR T1 - Effect of IAA and BAP Application in Varying Concentration on Zygotic Ovule Culture in Nigella sativa L. TT - Nigella sativa L.'da Zigotik Ovül Kültürü Üzerine Farklı Konsantrasyonlarda IAA ve BAP Uygulamasının Etkisi AU - Uysal, Hüseyin AU - Aydın Mortaş, Merve AU - Sevindik, Emre PY - 2025 DA - June Y2 - 2025 DO - 10.25308/aduziraat.1685595 JF - Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi JO - ADÜ ZİRAAT DERG PB - Aydın Adnan Menderes Üniversitesi WT - DergiPark SN - 1304-7787 SP - 145 EP - 150 VL - 22 IS - 1 LA - en AB - This study was carried out to test the effect of BAP (6-Benzylaminopurine) and IAA (Indole-3-acetic acid) hormones, both on embryo development in in vitro culture, and on obtaining hybrid plants of black cumin. Ovule culture was preferred in the study because the seeds were too small and embryo isolation was difficult. Ovule cultures were done by using populations obtained from producers in Ankara and Denizli in Türkiye and the Çameli variety. Hybridizations were done according to the semi-diallel hybridization method to gain zygotic embryos. MS (Duchefa Biochemie, Catalog No: M0222) mediums, with BAP or IAA (0.0, 0.5, 1.0, 2.0 mg/l) and BAP+IAA (1.0+1.0, 0.1+2.0, 2.0+0.5 mg/l) added, were used for ovule culture. As a result of the research, the highest success was gained for direct regeneration (1.7%) in the medium without hormone. The highest success gained for callus regeneration (2.3%) was in the medium having 2.0 mg/l BAP + 0.5 mg/l IAA added. The highest plant regeneration from callus was gained in the medium with an added 1.0 mg/l IAA as 7.7%. KW - Nigella sativa KW - Black cumin KW - Embryo culture KW - auxin KW - cytokinin N2 - Bu çalışma BAP (6-Benzylaminopurine) ve IAA (Indole-3-acetic acid) hormonlarının Çörek otu bitkisinde in vitro kültürde embriyo gelişimi üzerine etkisini araştırmak ve melez bitkiler elde etmek amacıyla gerçekleştirilmiştir. Tohumların çok küçük olması ve embriyo izolasyonunun zor olması nedeniyle çalışmada ovül kültürü tercih edilmiştir. Ovül kültürleri, Türkiye'de Ankara ve Denizli'deki üreticilerden elde edilen popülasyonlar ve Çameli çeşidi kullanılarak yapılmıştır. Melezlemeler, zigotik embriyolar elde etmek için yarı diallel melezleme yöntemine göre yapılmıştır. Ovül kültürü için BAP veya IAA (0.0, 0.5, 1.0, 2.0 mg/l) ve BAP+IAA (1.0+1.0, 0.1+2.0, 2.0+0.5 mg/l) eklenmiş MS (Duchefa Biochemie, Katalog No: M0222) besiyerleri kullanılmıştır. Araştırma sonucunda en yüksek başarı doğrudan rejenerasyonda (%1,7) hormonsuz ortamda elde edilmiştir. 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