TY  - JOUR
T1  - RESERPINE ENHANCES THE GENOTOXIC EFFECTS OF DOXORUBICIN IN HEPATOCELLULAR CARCINOMA CELLS
TT  - Reserpin Hepatosellüler Karsinom Hücrelerinde Doksorubisinin Genotoksik Etkilerini Artırır
AU  - Yurtcu, Erkan
AU  - Özdoğru, Sümeyra
AU  - Şengül, Sedef Nur
AU  - Sayın, Enes
AU  - Biçer, Beyza Nur
AU  - Arslan, Safa Burak
AU  - Bucak, Zülal
PY  - 2025
DA  - August
Y2  - 2025
DO  - 10.24938/kutfd.1693317
JF  - The Journal of Kırıkkale University Faculty of Medicine
JO  - Kırıkkale Uni Med J
PB  - Kırıkkale Üniversitesi
WT  - DergiPark
SN  - 2148-9645
SP  - 192
EP  - 196
VL  - 27
IS  - 2
LA  - en
AB  - Objective: Hepatocellular carcinoma is the most frequently diagnosed primary liver malignancy. Despite advances in treatment, challenges such as drug resistance limit the effectiveness of chemotherapy, including doxorubicin. Studies have shown that reserpine, a naturally occurring indole alkaloid, has anticancer properties in various malignancies. The aim of this study was to investigate whether reserpine increases the effectiveness of doxorubicin in hepatocellular carcinoma cells under in vitro conditions. Material and Methods: Human hepatocellular carcinoma (HepG2) cells were cultured. The IC50 value of reserpine was determined by the MTT method. Doxorubicin and reserpine were applied to HepG2 cells, both separately and in combination. DNA damage was evaluated by the alkaline comet assay. Results: Doxorubicin and reserpine significantly increased DNA damage in HepG2 cells when applied alone (p
KW  - Hepatocellular carcinoma
KW  - reserpine
KW  - doxorubicin
KW  - genotoxicity
N2  - Amaç: Primer karaciğer maligniteleri arasında en sık tanı konulanı hepatosellüler karsinomdur. Tedavideki ilerlemelere rağmen, ilaç direnci gibi zorluklar doksorubisin dahil olmak üzere kemoterapilerin etkinliğini sınırlandırmaktadır. Yapılan çalışmalarda doğal olarak oluşan bir indol alkaloidi olan reserpinin çeşitli malignitelerde antikanser özellikler göstermiştir. Bu çalışmada amacımız, in vitro koşullarda reserpinin hepatosellüler karsinom hücrelerinde doksorubisinin etkinliğini artırıp artırmadığı araştırılmasıdır. Gereç ve Yöntemler: İnsan hepatosellüler kanser hücreleri (HepG2) kültüre edildi. Rezerpinin IC50 değeri MTT testi ile belirlendi. Doksorubisin ve reserpin HepG2 hücrelerine hem ayrı ayrı hem de birlikte uygulandı. DNA hasarı ise alkali comet testi ile değerlendirildi. Bulgular: Doksorubisin ve rezerpin tek başına uygulandığında HepG2 hücrelerinde DNA hasarını anlamlı düzeyde artırdı (p
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UR  - https://doi.org/10.24938/kutfd.1693317
L1  - https://dergipark.org.tr/tr/download/article-file/4843834
ER  -