TY  - JOUR
T1  - Potential Efficiency of Aspergillus chevalieri Against Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) Larvae: Conidial Suspension and Ethanol Extract
TT  - Aspergillus chevalieri'nin Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) Larvalarına Karşı Potansiyel Etkinliği: Konidial Süspansiyon ve Etanol Ekstraktı
AU  - Güner, Pınar
AU  - Aşkun, Tülin
AU  - Er, Aylin
AU  - Deniz Sönmez, Görkem
AU  - Korkmaz, Raziye
PY  - 2025
DA  - September
Y2  - 2025
DO  - 10.31594/commagene.1709718
JF  - Commagene Journal of Biology
JO  - Comm. J. Biol.
PB  - ABADER (Adıyaman Bilimsel Araştırmalar Derneği)
WT  - DergiPark
SN  - 2602-456X
SP  - 198
EP  - 209
VL  - 9
IS  - 2
LA  - en
AB  - In recent years, there has been a notable increase in research data supporting the use of fungal species from diverse genera such as Penicillium, Aspergillus, Fusarium, Beauveria, Cordyceps, Metarhizium, and Purpureocillium in biological control applications. The current study was conducted to identify Aspergillus chevalieri using morphological characteristics and molecular data, then to determine the potential efficiency of conidial suspension and ethanol extract against Ephestia kuehniella and to investigate its mycotoxin production potential and cytotoxicity. The identification was carried out using phenotypic characteristics and sequences of the internal transcribed spacer (ITS), beta-tubulin gene (benA), and RNA polymerase II second largest subunit (RPB2) loci. In developmental biology studies, it was determined that topically applied conidial suspensions and ethanol extracts at varying concentrations affected different life stages of the insect. In the conidial suspension treatments, the larval period (at 10⁸ conidia/mL) and pupal period (at 10⁶, 10⁷, and 10⁸ conidia/mL) were notably shortened compared to the control group. In ethanol extract applications, the adult emergence time was reduced at the lowest concentrations (0.5 mg/mL and 1 mg/mL). Furthermore, both conidial suspensions and ethanol extracts caused a significant decrease in the total number of eggs, depending on the concentration applied. In the cytotoxicity test, the ethanol extract of the fungus was found to be cytotoxic in the L929 mouse cell line (NCTC clone 929) at concentrations above 0.78 mg/mL. This study showed that the fungus does not produce aflatoxin and ochratoxin and provided the first information on its potential efficiency against E. kuehniella larvae. Based on the present findings, A. chevalieri can be considered a promising candidate for inclusion in biological control programs. To fully assess its potential, future studies should explore its efficacy against a broader range of pest species and conduct field trials under diverse environmental conditions to validate the laboratory results.
KW  - Fungal identification
KW  - fungal pathogenicity
KW  - developmental biology
KW  - biological control
KW  - sustainable agriculture
N2  - Son yıllarda, Penicillium, Aspergillus, Fusarium, Beauveria, Cordyceps, Metarhizium ve Purpureocillium gibi çeşitli cinslere ait mantar türlerinin biyolojik mücadelede kullanılmasına yönelik araştırma verilerinde dikkate değer bir artış yaşanmıştır. Bu çalışma, Aspergillus chevalieri'nin morfolojik özellikleri ve moleküler veriler kullanılarak tanımlanması, ardından konidiyum süspansiyonu ve etanol ekstraktının Ephestia kuehniella'ya karşı potansiyel etkinliğinin belirlenmesi ve ayrıca mikotoksin üretim potansiyeli ile sitotoksisitesinin araştırılması amacıyla gerçekleştirilmiştir. Tanımlamada; fenotipik özelliklere ek olarak iç transkribe edilen aralık (ITS), beta-tübülin (benA) ve RNA polimeraz II'nin ikinci büyük alt birimi (RPB2) gen bölgelerinin dizileri kullanılmıştır. Gelişim biyolojisi çalışmalarında, topikal olarak uygulanan farklı dozlardaki konidiyal süspansiyonların ve etanol ekstraktlarının böceğin farklı gelişim evrelerinde etkili olduğu belirlendi. Konidiyal süspansiyon uygulamalarında özellikle kontrole göre larval periyodun (108 konidia/mL) ve pupal periyodun (106, 107, 108 konidia/mL) kısaldığı görülürken, etanol ekstresi uygulamalarında ise en düşük dozlarda ergin çıkış süresinin (0.5 mg/mL ve 1 mg/mL) kısaldığı görülmüştür. Ayrıca, hem konidial süspansiyonların hem de etanol ekstraktının konsantrasyonuna bağlı olarak kontrole göre yumurta sayısında önemli azalmalar görülmüştür. Sitotoksisite testinde ise fungusun etanol ekstraktı, 0.78 mg/mL üzerindeki konsantrasyonlarda L929 fare hücre hattı (NCTC clone 929) üzerinde sitotoksik etki göstermiştir. Çalışma ayrıca, bu mantarın aflatoksin ve okratoksin üretmediğini ortaya koymuş ve E. kuehniella larvalarına karşı potansiyel etkinliği konusunda ilk bilgileri sunmuştur. Elde edilen bulgulara dayanarak, A. chevalieri biyolojik mücadele programlarına dahil edilebilecek umut verici bir aday olarak değerlendirilebilir. Bu potansiyelin tam olarak anlaşılabilmesi için, gelecekte yapılacak araştırmalarda A. chevalieri’nin farklı zararlı türleri üzerindeki etkisinin değerlendirilmesi ve çeşitli çevresel koşullarda saha denemelerinin gerçekleştirilmesi gerekmektedir.
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ER  -