@article{article_1723791, title={Investigation of Drug Resistant Mycobacterium tuberculosis Strains with Molecular Methods}, journal={Journal of Uludağ University Medical Faculty}, volume={51}, pages={291–294}, year={2025}, DOI={10.32708/uutfd.1723791}, author={Payaslıoğlu, Ayşe Melda and Sağlam, Sezcan and Ak Aksoy, Seçil and Özakın, Cüneyt}, keywords={mycobacterium tuberculosis, drug resistance, moleküler analiz}, abstract={The aim of this study was to investigate mutations in the rpoB and embB genes using molecular methods in Mycobacterium tuberculosis isolates phenotypically resistant to rifampicin and/or ethambutol. Drug susceptibility testing of 110 isolates collected in Turkey between 2005 and 2014 was performed using the modified agar proportion method. Molecular analysis was conducted on 28 selected isolates, including 7 rifampicin-resistant strains. Mutations were detected within the Rifampicin Resistance-Determining Region (RRDR) between codons 507–533 of the rpoB gene. The most common mutation was Ser531Leu (TCG→TTG), found in 10 strains; one strain had a His526Asn (CAC→AAC) mutation. These mutations were considered important molecular markers of rifampicin resistance. Rapid molecular detection of these mutations is important for early and effective tuberculosis treatment planning.}, number={2}, publisher={Bursa Uludağ Üniversitesi}