TY  - JOUR
T1  - Ovarian Ischemia-Reperfusion Injury and the Potential Role of Mangiferin: A Histopathological, Biochemical Investigation
TT  - Over İskemi-Reperfüzyon Hasarı ve Mangiferin’in Olası Rolü: Histopatolojik ve Biyokimyasal Bir İnceleme
AU  - Ayaz, Hayat
AU  - Aşır, Fırat
PY  - 2025
DA  - September
Y2  - 2025
DO  - 10.30934/kusbed.1730683
JF  - Kocaeli Üniversitesi Sağlık Bilimleri Dergisi
JO  - KOU Sag Bil Derg
PB  - Kocaeli Üniversitesi
WT  - DergiPark
SN  - 2149-8571
SP  - 160
EP  - 166
VL  - 11
IS  - 3
LA  - en
AB  - Objective: Ischemia-reperfusion (IR) injury in the ovary can cause significant structural damage and compromise ovarian reserve by disrupting follicular integrity. This study aimed to investigate the protective effects of Mangiferin, a natural antioxidant and anti-inflammatory compound, in a rat model of ovarian IR injury. Histopathological evaluation, follicle counts, and serum anti-Müllerian hormone (AMH) levels were used to assess treatment efficacy.Methods: Thirty-two female Sprague-Dawley rats were divided into Control, Mangiferin, IR, and IR+ Mangiferin. Ovarian IR was induced by bilateral clamping of the ovarian vasculature for 3 hours, followed by 3 hours of reperfusion. Mangiferin (20 mg/kg) was administered intraperitoneally once daily for 15 days. Ovarian tissues were subjected to histological analysis with follicle counts and AMH levels were measured using ELISA.Results: The IR group exhibited marked histopathological changes, including vascular congestion, hemorrhage, edema, inflammatory cell infiltration, and extensive follicular degeneration. In contrast, the IR+ Mangiferin group showed improved tissue architecture, higher follicle counts, and AMH levels that were significantly preserved compared to the IR group.  Conclusion: Mangiferin may reduce ovarian damage caused by IR injury and contribute to the preservation of ovarian reserve. These findings suggest a potential therapeutic benefit of Mangiferin in protecting ovarian tissue under ischemic conditions.
KW  - Anti Müllerian Hormone
KW  - Histopathology
KW  - İschemia Reperfusion İnjury
KW  - Mangiferin
KW  - Ovary
N2  - Amaç: Overde gelişen iskemi-reperfüzyon (IR) hasarı, folikül bütünlüğünü bozarak belirgin doku hasarına ve over rezervinin azalmasına yol açabilir. Bu çalışmada, Mangiferin’in IR kaynaklı over hasarına karşı olası koruyucu etkilerinin; histopatolojik değişiklikler, folikül sayısı ve serum Anti-Müllerian Hormon (AMH) düzeyleri üzerinden değerlendirilmesi amaçlanmıştır.Yöntem: Otuz iki dişi Sprague-Dawley sıçan dört gruba ayrılmıştır: Kontrol, Mangiferin, IR ve IR+Mangiferin. Over IR modeli, bilateral over vaskülatürünün üç saat süreyle kleplenmesi ve ardından üç saatlik reperfüzyon uygulanması ile oluşturulmuştur. Mangiferin (20 mg/kg), 15 gün boyunca intraperitoneal yolla günde bir kez uygulanmıştır. Over dokuları histopatolojik incelemeye tabi tutulmuş; folikül sayımları gerçekleştirilmiş ve serum AMH düzeyleri ELISA yöntemiyle analiz edilmiştir.Bulgular: IR grubunda vasküler konjesyon, hemoraji, ödem, inflamatuar hücre infiltrasyonu ve belirgin folikül dejenerasyonu gibi ciddi histopatolojik bozulmalar gözlemlenmiştir. Buna karşın, IR+Mangiferin grubunda over dokusunun histolojik bütünlüğünün korunduğu, folikül sayılarının daha yüksek olduğu ve AMH düzeylerinin göreceli olarak korunduğu saptanmıştır.Sonuç: Mangiferin, IR kaynaklı over hasarını azaltabilir ve over rezervinin korunmasına katkı sağlayabilir. Bulgular, Mangiferin’in iskemik koşullarda over dokusunu korumaya yönelik potansiyel bir tedavi ajanı olabileceğini göstermektedir.
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UR  - https://doi.org/10.30934/kusbed.1730683
L1  - https://dergipark.org.tr/tr/download/article-file/5006011
ER  -