TY  - JOUR
T1  - Determination of the antioxidant activity and antibacterial effect of the α-lipoic acid-NHS molecule in fisheries products
TT  - Alfa-lipoik asit-NHS molekülünün antioksidan aktivitesinin ve su ürünlerindeki antibakteriyel etkisinin belirlenmesi
AU  - Kalkan, Samet
PY  - 2025
DA  - December
Y2  - 2025
DO  - 10.12714/egejfas.42.4.06
JF  - Ege Journal of Fisheries and Aquatic Sciences
JO  - EgeJFAS
PB  - Ege Üniversitesi
WT  - DergiPark
SN  - 2148-3140
SP  - 323
EP  - 330
VL  - 42
IS  - 4
LA  - en
AB  - Alpha-lipoic acid plays significant roles in metabolic pathways and provides substantial antioxidant defense. In this study, the N-hydroxysuccinimide ester derivative (α-lipoic acid–NHS) was examined for antioxidant performance and inhibitory effects on Gram-negative and Gram-positive bacterial strains isolated from aquatic environments. Antioxidant performance was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and CUPRAC (cupric ion reducing) assays. Additionally, The compound’s ability to mitigate oxidative stress was evaluated by quantifying reactive oxygen species (ROS) levels with the 8-OHdG ELISA method at different α-lipoic acid–NHS concentrations. Antibacterial activity was tested against four reference pathogens (Staphylococcus aureus, Aeromonas hydrophila, Salmonella enterica, Escherichia coli) and four environmental isolates (Bacillus cereus, B. toyonensis, Oceanisphaera sediminis, Pseudomonas putida) using the microdilution method. The results showed that α-lipoic acid–NHS exhibited negligible antioxidant activity in both DPPH and CUPRAC assays. In terms of antibacterial effects, however, complete inhibition of growth was observed in some bacterial strains at higher concentrations (5–10 mM). While ROS levels decreased modestly with increasing α-lipoic acid–NHS, this effect did not correlate with chemical antioxidant test results, suggesting the reduction was not due to direct antioxidant action. In conclusion, α-lipoic acid–NHS does not function as an effective direct radical scavenger but may exert indirect antioxidant effects and moderate antibacterial activity. It may have potential as a supportive compound in antioxidant systems and antibacterial therapies rather than as a standalone therapeutic agent.
KW  - Antibacterial effect
KW  - aquaculture
KW  - fish pathogen
KW  - minimum inhibitory concentration
KW  - oxidative stress
KW  - reactive oxygen species
N2  - Alfa-lipoik asit, metabolik yolaklarda önemli roller üstlenir ve güçlü bir antioksidan savunma sağlar. Bu çalışmada, α-lipoik asidin N-hidroksisüksinimid ester türevinin (α-lipoik asit–NHS) antioksidan etkinliği ve sucul ortamlardan izole edilen Gram-negatif ve Gram-pozitif bakteri suşlarında inhibitör aktivitesi değerlendirilmiştir. Antioksidan performans, DPPH (2,2-diphenyl-1-picrylhydrazyl) radikal süpürme ve CUPRAC (Cu2+ iyon indirgeme) testleri ile analiz edilmiştir. Ayrıca, farklı α-lipoik asit–NHS konsantrasyonlarında, 8-OHdG ELISA yöntemi ile reaktif oksijen türleri (ROS) düzeyleri ölçülerek bileşiğin oksidatif stresi baskılama potansiyeli incelenmiştir. Antibakteriyel aktivite, dört referans patojen (Staphylococcus aureus, Aeromonas hydrophila, Salmonella enterica, Escherichia coli) ve dört çevresel izolat (Bacillus cereus, B. toyonensis, Oceanisphaera sediminis, Pseudomonas putida) üzerinde mikrodilüsyon yöntemiyle test edilmiştir. Sonuçlar, α-lipoik asit–NHS’nin hem DPPH hem de CUPRAC testlerinde ihmal edilebilir düzeyde antioksidan aktivite gösterdiğini ortaya koymuştur. Buna karşılık, antibakteriyel etki açısından bakıldığında, bazı bakteri suşlarında yüksek konsantrasyonlarda (5–10 mM) tam büyüme inhibisyonu gözlenmiştir. Artan α-lipoik asit–NHS dozunda ROS düzeylerinde hafif bir azalma görülmüşsede, bu etki kimyasal antioksidan test sonuçlarıyla paralellik göstermemiştir; yani gözlenen azalmanın doğrudan antioksidan etkiden kaynaklanmadığı düşünülmektedir. Sonuç olarak, α-lipoik asit–NHS, etkili bir doğrudan serbest radikal süpürücü olarak işlev görmez; ancak dolaylı antioksidan etkiler ve orta düzeyde antibakteriyel aktivite gösterebilir. Tek başına tedavi edici bir ajan olmaktan ziyade, antioksidan sistemlerde ve antibakteriyel tedavilerde destekleyici bir bileşik olarak potansiyele sahiptir.
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UR  - https://doi.org/10.12714/egejfas.42.4.06
L1  - https://dergipark.org.tr/tr/download/article-file/5047462
ER  -