TY - JOUR T1 - The Effectiveness of Some Biostimulants Against Meloidogyne incognita in Tomato Plants TT - Domates Bitkilerinde Meloidogyne incognita’ya Karşı Bazı Biyostimülantların Etkinliği AU - Ulaş, Furkan AU - Duman, Nagihan AU - Gümüş, Serkan AU - Imren, Mustafa PY - 2026 DA - April Y2 - 2026 DO - 10.24180/ijaws.1741879 JF - Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi PB - Bolu Abant İzzet Baysal Üniversitesi WT - DergiPark SN - 2149-8245 SP - 31 EP - 38 VL - 12 IS - 1 LA - en AB - Root-knot nematodes (Meloidogyne spp.) are common plant pathogens that cause significant yield losses, particularly in vegetable crops such as tomatoes. Due to the environmental impacts and legal restrictions associated with chemical nematicides, the development and evaluation of biological alternatives have become increasingly important. In this study, the efficacy of four biological products and one chemical nematicide against Meloidogyne incognita was evaluated through a pot experiment conducted under growth room conditions. The biological products used were Biostimulant-I (Oxydez Plus), Biostimulant-II (Ozark), Biostimulant-III (Konsorta Root), and Biostimulant-IV (Accudo). The chemical nematicide was Velum Prime SC 400, which contains the active ingredient fluopyram. The efficacy of the products was examined based on the root-knot index and second-stage juvenile (J2) population parameters in a 70-day growth room experiment. The results showed that the chemical nematicide provided the highest efficacy, by reducing the root-knot index by 67.08% and the J2 population by 87.67%. Among the biological products, Biostimulant-IV suppressed root-knot formation by 43.88% and the J2 population by 51.74%. Biostimulant-III, applied at a high dose, also showed a significant effect, reducing root-knot formation by 32.46% and the J2 population by 40.71%. In contrast, Biostimulant-I and Biostimulant-II showed lower levels of effect, reducing the root-knot index by 12.63% and 10.97%, respectively, and the J2 population by 21.57% and 24.34%, respectively. These findings suggest that biological products could serve as an alternative to chemical products in controlling M. incognita when supported by appropriate doses and application timings. KW - Root-knot nematode KW - Meloidogyne incognita KW - Biological control KW - Biostimulant KW - Nematicide N2 - Kök-ur nematodları (Meloidogyne spp.), özellikle domates gibi sebze türlerinde ciddi verim kayıplarına yol açan yaygın bitki patojenlerindendir. Kimyasal nematisitlerin çevresel etkileri ve yasal kısıtlamalar nedeniyle, biyolojik alternatiflerin geliştirilmesi ve etkinliğinin değerlendirilmesi giderek daha fazla önem kazanmaktadır. Bu çalışmada, Meloidogyne incognita’ya karşı dört biyolojik ürün ile bir kimyasal nematisidin etkinliği, büyüme odası koşullarında yürütülen saksı denemesiyle değerlendirilmiştir. Biyolojik ürünler Biostimulant-I (Oxydez Plus), Biostimulant-II (Ozark), Biostimulant-III (Konsorta Root) ve Biostimulant-IV (Accudo) olarak kullanılmıştır. Kimyasal nematisit ise fluopyram etken maddesi içeren Velum Prime SC 400’dür. Ürünlerin etkinlikleri, 70 gün süren büyüme odası denemesinde kök-ur indeksi ve ikinci dönem larva (J2) sayısı parametreleri esas alınarak incelenmiştir. Elde edilen sonuçlar, kimyasal nematisidin kök-ur indeksini %67.08 ve J2 popülasyonunu %87.67 oranında azaltarak en yüksek etkinliği sağladığını ortaya koymuştur. Biyolojik ürünlerden Biostimulant-IV, kök-ur oluşumunu %43.88 ve J2 popülasyonunu %51.74 oranında baskılamıştır. Yüksek dozda uygulanan Biostimulant-III de sırasıyla %32.46 ve %40.71 oranlarında kayda değer bir etki göstermiştir. Buna karşılık, Biostimulant-I ve Biostimulant-II daha düşük düzeyde etki göstermiştir ve kök-ur indeksinde sırasıyla %12.63 ve %10.97, J2 popülasyonunda ise %21.57 ve %24.34 oranlarında azalma sağlamıştır. Bu bulgular, biyolojik ürünlerin uygun doz ve uygulama zamanlarıyla desteklenmesi durumunda M. incognita kontrolünde kimyasal ürünlere alternatif olabileceğini göstermektedir. CR - Abd-Elgawad, M. M. (2021). Optimizing safe approaches to manage plant-parasitic nematodes. Plants, 10(9), 1911. https://doi.org/10.3390/plants10091911 CR - Abokorah, M. S., & Fathalla, A. M. (2022). The nematicidal efficacy of fulvic acid, yeast fungus (Saccharomyces cerevisiae) and L-tryptophan on plant parasitic nematodes, growth, and yield of banana plants. Egyptian Journal of Crop Protection, 17(1), 27-37. https://doi.org/10.21608/ejcp.2022.132161.1005 CR - Ahmed, H., Gad, S., El Sherif, A., & El-Hadidy, E. (2022). Efficacy of five biopesticides for the management of root-knot nematode, Meloidogyne incognita infecting pepper (Capsicum annum L.) plants. Egyptian Journal of Agronematology, 21(1), 23-33. https://dx.doi.org/10.21608/ejaj.2022.218707 CR - Ali, A. A., Mahgoub, S. A., Ahmed, A. F., Mosa, W. F. A., El-Saadony, M. T., Mohamed, M. D. A., Alomran, M. M., Al-Gheffari, H. K., El-Tarabily, K. A., AbuQamar, S. F., & El-Ashry, R. M. (2024): Utilizing endophytic plant growth-promoting bacteria and the nematophagous fungus Purpureocillium lilacinum as biocontrol agents against the root-knot nematode (Meloidogyne incognita) on tomato plants. European Journal of Plant Pathology 170, 417–436. https://doi.org/10.1007/s10658-024-02907-z CR - Bridge, J., & Page, S. L. J. (1980). Estimation of root-knot nematode infestation levels on roots using a rating chart. International Journal of Pest Management, 26(3), 296-298. CR - Collinge, D. B., Jensen, D. F., Rabiey, M., Sarrocco, S., Shaw, M. W., & Shaw, R. H. (2022). Biological control of plant diseases–What has been achieved and what is the direction?. Plant Pathology, 71(5), 1024-1047. https://doi.org/10.1111/ppa.13555 CR - Engelbrecht, G., Horak, I., Jansen van Rensburg, P. J., & Claassens, S. (2018). Bacillus-based bionematicides: development, modes of action and commercialisation. Biocontrol Science and Technology, 28(7), 629-653. https://doi.org/10.1080/09583157.2018.1469000 CR - Goyat, N., Singh, S., Sharma, M. M. M., & Sharma, P. (2024). Biostimulants in sustainable management of phytoparasitic nematodes in plants. In Biostimulants in Plant Protection and Performance (pp. 319-348). Elsevier. https://doi.org/10.1016/B978-0-443-15884-1.00006-3 CR - Hammad, E. A., & El-Sagheer, A. M. (2023). Comparative efficacy of essential oil nanoemulsions and bioproducts as alternative strategies against root-knot nematode, and its impact on the growth and yield of Capsicum annuum L. Journal of the Saudi Society of Agricultural Sciences, 22(1), 47-53. https://doi.org/10.1016/j.jssas.2022.06.002 CR - Hamrouni, R., Regus, F., Farnet Da Silva, A. M., Orsiere, T., Boudenne, J. L., Laffont-Schwob, I., Cristen, P., & Dupuy, N. (2025). Current status and future trends of microbial and nematode-based biopesticides for biocontrol of crop pathogens. Critical Reviews in Biotechnology, 45(2), 333–352. https://doi.org/10.1080/07388551.2024.2370370 CR - Haque, Z., & Khan, M. R. (2022). Evaluation of fluopyram against single and concomitant inoculation of Rhizoctonia solani and Meloidogyne graminicola infesting rice crop. Phytoparasitica, 50(4), 811-822. https://doi.org/10.1007/s12600-022-01014-1 CR - Hooper, D. J. (1986). Extraction of nematodes from plant material (pp. 59–80). In J. F. Southey (Ed.), Laboratory methods for work with plant and soil nematodes (202 pp.). Ministry of Agriculture, Fisheries and Food. CR - Kariuki, G. M., Brito, J. A., & Dickson, D. W. (2006). Rate of attachment of Meloidogyne arenaria by Pasteuria penetrans affects root penetration and nematode fecundity. Nematropica, 36, 261–267. CR - Kepenekci, L., Dura, O., & Dura, S. (2017). Determination of nematicidal effects of some biopesticides against root-knot nematode (Meloidogyne incognita) on Kiwifruit. Journal of Agricultural Science and Technology, 7, 546-551. https://doi.org/10.17265/2161-6256/2017.08.004 CR - Kiewnick, S., & Sikora, R. A. (2006). Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biological Control, 38(2), 179-187. https://doi.org/10.1016/j.biocontrol.2005.12.006 CR - Le Mire, G., Nguyen, M. L., Fassotte, B., du Jardin, P., Verheggen, F., Delaplace, P., & Jijakli, H. (2016). Implementing plant biostimulants and biocontrol strategies in the agroecological management of cultivated ecosystems: A review. Biotechnologie, Agronomie, Société et Environnement, 20, 299–313. CR - Mitkowski, N., & Abawi, G. (2003). Reproductive fitness on lettuce of populations of Meloidogyne hapla from New York State vegetable fields. Nematology, 5(1), 77-83. CR - Mukhtar, T., Kayani, M. Z., & Hussain, M. A. (2013). Response of selected cucumber cultivars to Meloidogyne incognita. Crop Protection, 44, 13–17. https://doi.org/10.1016/j.cropro.2012.10.015 CR - Nicol, J. M., Turner, S. J., Coyne, D. L., Den Nijs, L., Hockland, S., & Maafi, Z. T. (2011). Current nematode threats to world agriculture. In J. Jones, G. Gheysen, & C. Fenoll (Eds.), Genomics and molecular genetics of plant-nematode interactions (pp. 21–43). Springer. https://doi.org/10.1007/978-94-007-0434-3_2 CR - Noling, J. W. (2014). Diseases caused by nematodes. In J. B. Jones, T. W. Zitter, T. M. Momol, & S. A. Miller (Eds.), Compendium of tomato diseases and pests (pp. 113–119). The American Phytopathological Society. CR - Onkendi, E. M., Kariuki, G. M., Marais, M., & Moleleki, L. N. (2014). The threat of root‐knot nematodes (Meloidogyne spp.) in A frica: a review. Plant Pathology, 63(4), 727-737. https://doi.org/10.1111/ppa.12202 CR - Özevin, F. N., Ulaş, F., Imren, M., Güvenç, C., & Yüksel, E. (2025). Optimization of a Bacillus-based bioproduct for Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) control using response surface methodology. Turkish Journal of Entomology, 49(2), 187-200. https://doi.org/10.16970/entoted.1667614 CR - Perry, R. N., Moens, M., & Starr, J. L. (Eds.). (2009). Root-knot nematodes (1st ed.). CABI. CR - Radwan, M. A., Farrag, S. A. A., Abu-Elamayem, M. M., & Ahmed, N. S. (2012). Biological control of the root-knot nematode, Meloidogyne incognita on tomato using bioproducts of microbial origin. Applied Soil Ecology, 56, 58-62. https://doi.org/10.1016/j.apsoil.2012.02.008 CR - Saad, A. F. S., Massoud, M. A., Ibrahim, H. S., & Khalil, M. S. (2012). Activity of nemathorin, natural product and bioproducts against root-knot nematodes on tomatoes. Archives of Phytopathology and Plant protection, 45(8), 955-962. https://doi.org/10.1080/03235408.2012.655145 CR - Sikora, R. A., & Fernandez, E. (2005). Nematode parasites of vegetables. In M. Luc, R. A. Sikora, & J. Bridge (Eds.), Plant parasitic nematodes in subtropical and tropical agriculture (pp. 319–392). CABI. https://doi.org/10.1079/9780851997278.0319 CR - Xiong, J., Zhou, Q., Luo, H., Xia, L., Li, L., Sun, M., & Yu, Z. (2015). Systemic nematicidal activity and biocontrol efficacy of Bacillus firmus against the root-knot nematode Meloidogyne incognita. World Journal of Microbiology and Biotechnology, 31, 661-667. https://doi.org/10.1007/s11274-015-1820-7 CR - Yılmaz, İ., Ulaş, F., & Imren, M. (2025). The effect of some biopesticides on the root-knot nematode, Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) damaging tomato plants. Turkish Journal of Entomology, 49(1), 19-26. https://doi.org/10.16970/entoted.1621229 UR - https://doi.org/10.24180/ijaws.1741879 L1 - https://dergipark.org.tr/tr/download/article-file/5052994 ER -