@article{article_1751517, title={Spectrochemical evidence for the reversal of age-related testicular degeneration by young plasma in senile infertility}, journal={Anatolian Current Medical Journal}, volume={7}, pages={760–769}, year={2025}, DOI={10.38053/acmj.1751517}, author={Teker, Hikmet Taner and Ceylani, Taha and Şengil, Ahmet Zeki}, keywords={Senil infertilite, testis dokusu, genç plazma, FTIR ile moleküler profil, spektroskopi, PCA modellemesi}, abstract={Aims: Senile infertility in males is primarily associated with testicular degeneration, although its precise etiology remains unclear. In recent years, increasing attention has been directed toward strategies aimed at ameliorating age-related physiological deterioration. The present study investigates the potential restorative effects of heterochronic blood plasma exchange between aged and young Wistar rats on the molecular composition of testicular tissue specifically targeting protein, lipid, carbohydrate, and nucleic acid profiles. Methods: Spectral data were acquired via fourier transform infrared (FTIR) spectroscopy and evaluated through principal component analysis (PCA). Results: Compared to the spectral characteristics of young testicular tissue, aged samples exhibited prominent molecular alterations indicative of structural degeneration, including spectral shoulder formation, band broadening, and frequency shifts—particularly associated with β-sheet-rich protein conformations. Notably, phospholipid and glycolipid-associated bands were present in aged controls and young rats receiving aged plasma, but absent in young controls and aged rats treated with young plasma. Similarly, spectral features related to testicular glycogen, methyl rocking, and nucleic acid phosphate groups were lost in aged tissues but preserved in young and rejuvenated aged samples. These alterations were statistically significant (p <0.01, 99% confidence). Conclusion: Overall, plasma exchange induced spectroscopically detectable modifications that reflect donor-specific molecular signatures in recipient testicular tissue. Restoration of protein secondary structures and reappearance of nucleic acid and glycogen-associated signals in aged rats receiving young plasma underscore the therapeutic potential of this intervention against male reproductive aging. Nevertheless, as this study was designed as an FTIR-centered spectrochemical analysis, it does not address deeper molecular layers such as proteomics or RNA-seq, the durability of plasma-induced effects over longer periods, or the potential applicability to the female reproductive system. Future studies are warranted to explore these dimensions and further strengthen translational relevance.}, number={6}, publisher={MediHealth Academy Yayıncılık}