@article{article_1752081, title={Receptor gene expressions and their association with biochemical parameters in primary hyperparathyroidism patients}, journal={Anatolian Current Medical Journal}, volume={7}, pages={607–612}, year={2025}, DOI={10.38053/acmj.1752081}, author={Acar, Özge}, keywords={Kalsiyum algılayıcı reseptör (CaSR), PTH, D vitamini reseptörü (VDR), Fibroblast büyüme faktörü reseptörü 1 (FGFR1), α-Klotho, Primer hiperparatiroidizm (PHPT)}, abstract={Aims: Primary hyperparathyroidism (PHPT) is recognized by excessive parathyroid hormone (PTH) secretion, leading to hypercalcemia and systemic complications. This study aimed to investigate the gene expression profiles of PTH, calcium-sensing receptor (CaSR), vitamin D receptor (VDR), fibroblast growth factor receptor 1 (FGFR1), and α-Klotho from parathyroid adenomas among PHPT patients and to explore their correlations with biochemical parameters. Methods: Parathyroid adenoma samples were obtained from 20 PHPT patients who underwent parathyroidectomy. RNA was isolated, and gene expression levels were quantified using one-step reverse transcription quantitative PCR (RT-qPCR). Fold change values were calculated utilizing gene-specific patient average ΔCt values as references. Biochemical data, including serum calcium, phosphorus, PTH, and vitamin D (25-OH), as well as gender, age, and adenoma volumes, were statistically analyzed for correlation with gene expression patterns. Results: Gene expression analysis revealed significantly lower CaSR expression in small adenomas ( <0.2 cm³) compared to medium and large adenomas, suggesting a possible association between tumor size and early molecular deregulation. Notably, CaSR expression exhibited a positive correlation with the expression levels of both VDR and FGFR1, suggesting the presence of a coordinated regulatory network encompassing calcium and vitamin D pathways. A multiple regression model revealed that serum vitamin D and PTH gene expression serve as positive predictors of CaSR expression, whereas serum PTH levels were negatively associated. A paradoxical positive correlation was observed between serum calcium levels and PTH gene expression, which may reflect impaired feedback control due to CaSR downregulation. Conclusion: These findings may provide new insights into the pathophysiology of PHPT, highlighting the importance of molecular profiling in understanding disease progression. The established expression patterns and their biochemical correlations may strengthen future efforts toward population-specific diagnosis and targeted therapeutic approaches, particularly in regions with high vitamin D deficiency and distinct disease severity profiles.}, number={5}, publisher={MediHealth Academy Yayıncılık}, organization={This work was supported by Istanbul Okan University Scientific Research Projects Coordination under the Grant Number OBAP2024010002.}