TY  - JOUR
T1  - Hayvansal Gıdalardan İzole Edilen Escherichia coli ve Klebsiella spp. Bakterilerinin Genişletilmiş Spektrumlu Βeta Laktamaz Üretimi Ve Çoklu Antibiyotik Dirençliliğinin Belirlenmesi
AU  - Beyzi, Ebru
AU  - Yılmaz, Adem
AU  - Külahcı, Meryem Burcu
AU  - Çıtak, Sumru
PY  - 2025
DA  - November
Y2  - 2025
DO  - 10.63716/guffd.1795977
JF  - Gazi Üniversitesi Fen Fakültesi Dergisi
JO  - GÜFFD
PB  - Gazi Üniversitesi
WT  - DergiPark
SN  - 2757-5543
SP  - 330
EP  - 342
VL  - 6
IS  - 2
LA  - tr
AB  - Antibiyotiklerin, veterinerlikte büyüme faktörü olarak, profilakside ve tedavide sıklıkla kullanılması, dirençli bakterilerin hayvansal gıdalar aracılığıyla insanlara geçişini artırmaktadır. Bu durum toplum sağlığı açısından sorunlara neden olmaktadır. Çalışmada Ankara'da satışa sunulan 26 adet peynir, 24 adet çiğ süt, 8 tavuk ve 13 et örneğinden izole edilen Enterobacterales izolatlarının tür düzeyinde tanımlamaları MALDI-TOF-MS ile gerçekleştirilmiştir. İdentifikasyon sonrasında toplam 147 Enterobacterales izolatının 44’ü (%29,9) E. coli, 33’ü (%22,5) Klebsiella pneumoniae, 23’ü (%15,6) Citrobacter spp, 15’i (%10,2) Enterobacter cloacae, 6’sı (%4,1) Raoultella ornithinolytica, 4’ü (%2,7) Enterobacter ludwigii, 3’ü (%2) Klebsiella oxytoca, 2’şer tanesi (%1,3) Enterobacter hormaechei, Enterobacter kobei, Buttiauxella agrestis ve Leclercia adecarboxylata ve 1’er tanesi (%0,7) de Serratia liquefaciens ve Acinetobacter baumannii olarak adlandırılmıştır. Enterobacterales üyelerinin antibiyotik dirençlilik durumu Kirby-Bauer Disk Difüzyon yöntemi, Genişletilmiş Spektrumlu Beta-Laktamaz (GSBL) çift disk sinerji testi ve CHROMagar ESBL'de koloni rengi ile araştırılmıştır. Çift disk sinerji testinde (ÇDST) toplam 147 Enterobacterales izolatının 81'i (%55,1) GSBL pozitif, 66'sı (%44,9) GSBL negatif bulunmuştur. Kromojenik agar GSBL testinde ise 147 Enterobacterales izolatının 67'si (%45,6) GSBL pozitif, 80'i (%54,4) GSBL negatif bulunmuştur. 44 E. coli izolatının 25'i (%56), 36 Klebsiella izolatının 24'ü (%66) GSBL pozitif olarak belirlenmiştir. Enterobacterales izolatları en fazla (%53,7) ampisilin ve (%32,7) amoksisilin klavulanik asite dirençli bulunmuştur. İzolatların tamamı (%100) amikasin ve imipeneme duyarlı bulunmuştur.
KW  - Escherichia coli
KW  - Klebsiella spp.
KW  - GSBL
KW  - Antibiyotik direnci
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UR  - https://doi.org/10.63716/guffd.1795977
L1  - https://dergipark.org.tr/tr/download/article-file/5296025
ER  -