@article{article_1798314, title={Investigation of the presence of nervous necrosis virus (NNV) in pikeperch (Sander lucioperca) and crayfish (Pontastacus leptodactylus)}, journal={Ege Journal of Fisheries and Aquatic Sciences}, volume={43}, pages={1–8}, year={2026}, DOI={10.12714/egejfas.43.1.01}, url={https://izlik.org/JA33UE33RN}, author={Eren, Hakan and Yavru, Sibel}, keywords={Betanodavirus, yabani balık, tatlı su, RT-PCR, E-11 hücre hattı}, abstract={In this study, samples were taken from pikeperch (Sander lucioperca) and crayfish (Pontastacus leptodactylus) living in Eğirdir, Beyşehir, and Suğla lakes, which are key stopovers for migratory birds. In 2022, crayfish and pikeperch were sampled from Eğirdir, Beyşehir and Suğla lakes at 3 different water temperature periods (15-20°C, 20-25°C and 25-30°C). A total of 540 samples (270 pikeperch and crayfish) were analyzed for the presence of NNV genome (RNA-1 and RNA2) by real-time RT-PCR and also inoculated in E-11 cell. No positive samples were recorded by any of the techniques. In this study, the presence of NNV was investigated for the first time in wild aquatic organisms living in freshwaters of Türkiye and NNV was not detected in the sampled pikeperch and crayfish. However, it is important to regularly examine these lakes, which are located on the migration routes of migratory birds, for NNV.}, number={1}, organization={This research was supported and funded by the General Directorate of Agricultural Research and Policies (TAGEM), Ministry of Agriculture and Forestry of the Republic of Türkiye, under Project No: TAGEM/HSGYAD/B/22/A5/P4/5421. The study is also derived from the doctoral dissertation submitted by Hakan Eren to the Institute of Health Sciences, Selçuk University.}