TY  - JOUR
T1  - Investigation of the presence of nervous necrosis virus (NNV) in pikeperch (Sander lucioperca) and crayfish (Pontastacus leptodactylus)
TT  - Sudak (Sander lucioperca) ve kerevitte (Pontastacus leptodactylus) nervöz nekrozis virüsünün (NNV) varlığının araştırılması
AU  - Eren, Hakan
AU  - Yavru, Sibel
PY  - 2026
DA  - February
Y2  - 2025
DO  - 10.12714/egejfas.43.1.01
JF  - Ege Journal of Fisheries and Aquatic Sciences
JO  - EgeJFAS
PB  - Ege Üniversitesi
WT  - DergiPark
SN  - 2148-3140
SP  - 1
EP  - 8
VL  - 43
IS  - 1
LA  - en
AB  - In this study, samples were taken from pikeperch (Sander lucioperca) and crayfish (Pontastacus leptodactylus) living in Eğirdir, Beyşehir, and Suğla lakes, which are key stopovers for migratory birds. In 2022, crayfish and pikeperch were sampled from Eğirdir, Beyşehir and Suğla lakes at 3 different water temperature periods (15-20°C, 20-25°C and 25-30°C). A total of 540 samples (270 pikeperch and crayfish) were analyzed for the presence of NNV genome (RNA-1 and RNA2) by real-time RT-PCR and also inoculated in E-11 cell. No positive samples were recorded by any of the techniques. In this study, the presence of NNV was investigated for the first time in wild aquatic organisms living in freshwaters of Türkiye and NNV was not detected in the sampled pikeperch and crayfish. However, it is important to regularly examine these lakes, which are located on the migration routes of migratory birds, for NNV.
KW  - Betanodavirus
KW  - wild fish
KW  - freshwater
KW  - RT-PCR
KW  - E-11 cell line
N2  - Bu çalışmada, göçmen kuşlar için önemli durak noktaları olan Eğirdir, Beyşehir ve Suğla göllerinde yaşayan sudak (Sander lucioperca) ve kerevitlerden (Pontastacus leptodactylus) NNV varlığı araştırılmıştır. 2022 yılında Eğirdir, Beyşehir ve Suğla göllerinden 3 farklı su sıcaklığı döneminde (15-20°C, 20-25°C ve 25-30°C) kerevit ve sudak örnekleri alınmıştır. Toplam 540 örnek (270 sudak ve kerevit) gerçek zamanlı RT-PCR ile NNV genomunun (RNA-1 ve RNA2) varlığı açısından analiz edilmiş ve ayrıca E-11 hücresine inokule edilmiştir. Hiçbir teknikle pozitif örnek kaydedilmemiştir. Bu çalışmada, Türkiye'nin tatlı sularında yaşayan yabani sucul organizmalarda NNV'nin varlığı ilk kez araştırılmış ve örneklenen sudak ve kerevitlerde NNV tespit edilmemiştir. Çalışmanın yapıldığı tarihte virusa rastlanılmamış olsada, göçmen kuşların göç yolları üzerinde bulunan bu göllerin NNV açısından düzenli olarak incelenmesinin önemli olduğu düşünülmektedir.
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UR  - https://doi.org/10.12714/egejfas.43.1.01
L1  - https://dergipark.org.tr/tr/download/article-file/5306866
ER  -