TY  - JOUR
T1  - Termostabil ve Asidik Selülazın Karakterizasyonu ve Meyve Suyu Berraklaştırılmasında Kullanımı
AU  - Arabacı, Nihan
PY  - 2025
DA  - November
Y2  - 2025
DO  - 10.63716/guffd.1799900
JF  - Gazi Üniversitesi Fen Fakültesi Dergisi
JO  - GÜFFD
PB  - Gazi Üniversitesi
WT  - DergiPark
SN  - 2757-5543
SP  - 275
EP  - 287
VL  - 6
IS  - 2
LA  - tr
AB  - Bu çalışmada, elma, üzüm ve şeftali sularının berraklaştırılmasında mikrobiyal selülaz enziminin kullanılma potansiyeli araştırılmıştır. Bu amaçla, Bacillus sp. S35 suşundan selülaz enzimi izole edilmiştir. Enzimin endüstriyel potansiyelini belirlemek amacıyla karakterizasyonu yapılmıştır. S35 selülaz, pH 5,0’te ve 40°C’de optimum aktivite göstermiştir. Enzim, pH 4,0-10,0 ve 25-60°C aralıklarında oldukça stabil olup asit-alkali ve termostabil karakter sergilemiştir. Ca+2, Cu+2, Mg+2 ve Zn+2 iyonlarına maruz kaldığında aktivitesini, sırasıyla, %89, %82, %88 ve %89 oranlarında koruyan selülazın aktivitesi, Co+2 ve Mn+2 iyonlarının varlığında %134 ve %130’a yükselmiştir. S35 selülaz, %0,02 β-merkaptoetanol ve 1 mM iyodoasetamid inhibitörlerinin varlığında aktivitesinin tamamını korumuştur. 1 mM EDTA varlığında %28 oranında aktivite kaybetmiştir. Enzim, %10’luk aseton, asetonitril, n-bütanol, etanol, gliserol, izopropanol ve metanol varlığında, sırasıyla, %89, %77, %70, %84, %64, %80 ve %77 oranlarında stabil kalmıştır. Meyve suyu berraklaştırılması analizinde yapılan geçirgenlik ölçümüne göre, enzim en iyi berraklık oranını (%C) şeftali suyundaki aktivitesi (%1421) sonucunda göstermiştir. İndirgen şeker miktarı analizinde de en yüksek verim 3 saatlik inkübasyon sonucunda şeftali suyunda elde edilmiştir. Elde edilen bulgular, S35 selülaz enziminin, meyve sularının berraklaştırılması amacıyla içecek endüstrisi proseslerinde yer alabilecek potansiyel bir aday olduğunu göstermektedir.
KW  - Selülaz
KW  - Bacillus sp.
KW  - Meyve suyu berraklaştırma
KW  - Transmitans
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UR  - https://doi.org/10.63716/guffd.1799900
L1  - https://dergipark.org.tr/tr/download/article-file/5313970
ER  -