TY - JOUR T1 - Identification, characterization and draft genome analysis of Vibrio anguillarum VK1 isolated from hatchery water TT - Kuluçkahane suyundan izole edilen Vibrio anguillarum VK1'in identifikasyonu, karakterizasyonu ve taslak genom analizi AU - Yaşa, İhsan AU - Toklu, Kadriye PY - 2026 DA - June Y2 - 2026 DO - 10.12714/egejfas.43.3.04 JF - Ege Journal of Fisheries and Aquatic Sciences JO - EgeJFAS PB - Ege Üniversitesi WT - DergiPark SN - 2148-3140 SP - 216 EP - 225 VL - 43 IS - 3 LA - en AB - Aquaculture environments are dynamic ecosystems that may influence the evolution and adaptive potential of bacterial pathogens. In this study, a locally isolated Vibrio anguillarum strain VK1 (PRJNA1452808) from Türkiye was characterized using 16S rDNA phylogenetic analysis and whole-genome-based approaches. The high-quality draft genome (4.23 Mb; GC content 44.31%) comprised 194 contigs and 4,102 coding sequences, including 1,057 hypothetical proteins. Functional analyses revealed putative stress response mechanisms and iron acquisition systems, suggesting metabolic versatility and environmental adaptability. Genome-based screening identified 33 putative antimicrobial resistance-associated determinants, including multidrug efflux-related genes and genes linked to antibiotics commonly used in aquaculture. In addition, genes associated with Type III and Type VI secretion systems were detected, potentially indicating adaptive traits related to host interaction and environmental persistence. Overall, the findings suggest that strain VK1 possesses a flexible genomic structure that may support adaptation to fluctuating aquaculture conditions. However, further functional studies are needed to biologically validate the potential virulence and AMR related features. KW - Aquaculture KW - vibriosis KW - next generation sequencing KW - virulence factors N2 - Su ürünleri yetiştiriciliği ortamları bakteriyel patojenlerin evrimini ve adaptasyon potansiyelini etkileyebilecek dinamik ekosistemlerdir. Bu çalışmada, Türkiye'den yerel olarak izole edilen Vibrio anguillarum VK1 suşu (PRJNA1452808), 16S rDNA filogenetik analizi ve tüm genom tabanlı yaklaşımlar kullanılarak karakterize edilmiştir. Yüksek kaliteli taslak genom (4,23 Mb; GC içeriği %44,31), 194 kontig ve 4.102 kodlama dizisi içermekte olup, bunların arasında 1.057 hipotetik protein bulunmaktadır. Fonksiyonel analizler, olası stres yanıt mekanizmalarını ve demir edinim sistemlerini ortaya çıkararak metabolik çok yönlülük ve çevresel adaptasyon yeteneğini işaret etmektedir. Genom tabanlı tarama, çoklu ilaç atılımı ile ilgili genler ve su ürünleri yetiştiriciliğinde yaygın olarak kullanılan antibiyotiklerle bağlantılı genler de dahil olmak üzere 33 olası antimikrobiyal dirençle ilişkili belirleyiciyi tanımlamıştır. Ayrıca, konakçı etkileşimi ve çevresel kalıcılıkla ilgili adaptif özellikleri potansiyel olarak gösteren Tip III ve Tip VI salgılama sistemleriyle ilişkili genler tespit edilmiştir. Genel olarak, bulgular VK1 suşunun, değişken akuakültür ortamlarına adaptasyonu destekleyebilecek esnek bir genomik yapıya sahip olduğunu göstermektedir. Bununla birlikte, olası virülans ve AMR ile ilişkili özelliklerin biyolojik doğrulaması için daha fazla fonksiyonel çalışmaya ihtiyaç duyulmaktadır. CR - AlJindan, R., AlEraky, D.M., Farhat, M., Almandil, N.B., AbdulAzeez, S., & Borgio, J.F. (2023). Genomic insights into virulence factors and multi-drug resistance in Clostridium perfringens IRMC2505A. 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