@article{article_19407, title={An investigation of mutations (FecXG , FecXI , FecXH , FecXB ) on BMP-15 gene in some local sheep breeds raised in Turkey}, journal={Akdeniz University Journal of the Faculty of Agriculture}, volume={25}, pages={29–33}, year={2012}, author={Karslı, T. and Şahin, E. and Karslı, B. Argun and Alkan, S. and Balcıoğlu, M. S.}, keywords={BMP-15 geni, Çoklu doğum, Koyun}, abstract={<p>Genetic mutations on major genes increase ovulation rate and litter size in sheep. Three major genes have been identified belonging to the TGF-β superfamily until now. These genes are <br />BMPR-IB (Bone morphogenetic protein receptor IB), BMP-15 (Bone morphogenetic protein15) and GDF9 (Growth differentiation factor 9). Different mutations (FecXB, FecXG, FecXI <br />,FecXH, FecXL and FecXR) on Bone Morphogenetic Protein-15 gene either increase ovulation rate or cause infertility in sheeps. The purpose of this study was to investigate whether the four different mutations (FecXG, FecXI, FecXH and FecXB) exist on BMP15 gene in five local sheep breeds reared in Turkey. PCR-RFLP method was used to determine these mutations. A total of 96 blood samples were investigated from Akkaraman (24 samples), Dağlıç (19 samples), İvesi (19 samples), Tuj (15 samples) and Karakaş (19 samples) sheep breeds. To determine FecXG, FecXI, FecXH and FecXB mutations, DNA fragments with 141, 154, 240 and 153 bp sizes were PCR amplified by using spesific primers, respectively. PCR products were digested to detect FecXG, FecXI, FecXH, FecXB point mutation by using HinfI, XbaI, AhlI, BstDEI restriction enzymes, respectively. As a result, FecXI, FecXH, FecXG and FecXB loci could not be <br />determined. Random selected 96 sheeps from five flocks were monomorph in terms of FecXI, FecXH, FecXG and FecXB loci. <br /> </p>}, number={1}, publisher={Akdeniz Üniversitesi}