TY  - JOUR
T1  - Tenebrio molitor larvae meal as fish meal alternative: impacts in juvenile narrow-clawed crayfish (Pontastacus leptodactylus)
TT  - Balık ununa alternatif olarak Tenebrio molitor larva unu: Jüvenil dar kıstkaçlı kerevitlerdeki (Pontastacus leptodactylus) etkileri
AU  - Özdoğan, Hasan Batuhan Emre
AU  - Uzunmehmetoğlu, Oğuz Yaşar
AU  - Bahadır Koca, Seval
AU  - Özmen, Özlem
AU  - Erol, Kamile Gonca
AU  - Koca, Habil Uğur
AU  - Öztuna, İsmail Hakkı
AU  - Akın, Mustafa
PY  - 2026
DA  - September
Y2  - 2026
DO  - 10.35229/jaes.1977607
JF  - Journal of Anatolian Environmental and Animal Sciences
JO  - JAES
PB  - Bülent VEREP
WT  - DergiPark
SN  - 2548-0006
VL  - 0
IS  - 2026
LA  - en
AB  - A 60-day trial examined replacing fish meal (FM) protein with defatted Tenebrio molitor (TM) larvae meal in diets for juvenile narrow-clawed crayfish (Pontastacus leptodactylus). Six isonitrogenous, isoenergetic diets with 0–100% FM protein replacement were tested on 600 stage II juveniles (four replicates of 25 per treatment).Growth performance was similar between the control and 40% TM groups (final weight and SGR did not differ significantly, p>0.05), but replacement levels above 40% significantly reduced growth and survival, with the 100% TM group showing the lowest final weight and survival (48%). Molting frequency and cheliped injury did not differ across treatments. Histological analysis showed normal hepatopancreas and intestine in the control and 20% TM groups. At ≥40% TM inclusion, hepatopancreatic damage (pigment deposits, elevated B-cell counts, degeneration, necrosis) and progressive shortening of intestinal villi were observed, along with luminal content stasis. Overall, up to 20% FM replacement with TM larvae meal can be used safely without harming growth or gut/hepatopancreas health, while higher inclusion levels risk long-term digestive tissue damage.
KW  - Tenebrio molitor
KW  - Crayfish
KW  - fish meal replacement
KW  - growth performance
KW  - histopathology
N2  - Bu 60 günlük çalışmada, balık ununun (BU) proteininin yağı alınmış Tenebrio molitor (TM) larva unu ile ikame edilmesinin, juvenil kerevit (Pontastacus leptodactylus) diyetlerindeki etkileri incelenmiştir. %0-100 oranında BU ikamesi içeren altı izonitrojenik ve izoenerjetik diyet, 600 evre II juvenil üzerinde test edilmiştir (her grupta 25'erli dört tekrar). Kontrol ve %40 TM grupları arasında büyüme performansı benzer bulunmuştur (final ağırlık ve SGR farkı anlamlı değil, p>0,05); ancak %40'ın üzerindeki ikame oranları büyüme ve yaşama oranını önemli ölçüde azaltmış, en düşük değerler %100 TM grubunda (yaşama oranı: %48) görülmüştür. Kabuk değiştirme sıklığı ve kıskaç yaralanması gruplar arasında farklılık göstermemiştir. Histolojik incelemede kontrol ve %20 TM gruplarında hepatopankreas ve bağırsak yapısı normal bulunmuştur. %40 TM ve üzerinde hepatopankreas hasarı (pigment birikimi, artmış B-hücre sayısı, dejenerasyon, nekroz) ve bağırsak villuslarında kademeli kısalma gözlenmiş, lümen içeriğinde staz eşlik etmiştir. Sonuç olarak, BU'nun TM larva unu ile %20'ye kadar ikamesi büyüme veya bağırsak/hepatopankreas sağlığını bozmadan güvenle kullanılabilirken, daha yüksek oranlar uzun vadede sindirim dokusu hasarı riski taşımaktadır.
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UR  - https://doi.org/10.35229/jaes.1977607
L1  - https://dergipark.org.tr/tr/download/article-file/6141900
ER  -