@article{article_210929, title={Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae)}, journal={Nevşehir Bilim ve Teknoloji Dergisi}, volume={3}, pages={33–47}, year={2015}, DOI={10.17100/nevbiltek.210929}, author={Sevim, Ali and Sevim, Elif and Demir, İsmail and Demirbağ, Zihni}, keywords={Yaprak kıvrım böceği, Beauveria bassiana, mikrobiyal mücadele}, abstract={<p style="text-align:justify;">The leafroller weevil beetle ( <em>Rhynchites bacchus </em> L., Coleoptera: Rhynchitidae) is one of the most destructive pest of various fruits such as apple, plum, apricot, cherry and peach (nectarine) etc. almost all over the world. This pest feeds either stone or pome fruits and can cause up to 80-90% damage to orchards. In this study, we investigated fungal pathogens of this pest to find more effective and safe biological control agent against it. Insect specimens were collected from apple orchards invaded by <em>R. baccus </em> during July of 2007 in Gümüşhane, Turkey. One fungal species was isolated and characterized by currently used morphological and molecular techniques including ITS and the partial EF1-α sequencing. Based on these characteristics, the isolate was identified as <em>Beauveria bassiana </em>. Separately, the insecticidal activity of the isolate was determined on <em>R. bacchus </em> adults. The fungus caused 100% mortality within two weeks days after application of 1 × 10 <sup>8 </sup> ml <sup>-1 </sup> spore concentration and the LT <sub>50 </sub> value of the fungus was calculated as 7.5 day. This is the first study to determine the effectiveness of <em>B. bassiana </em> against <em>R. baccus </em>. </p>}, number={2}, publisher={Nevşehir Hacı Bektaş Veli Üniversitesi}