@article{article_243749, title={Anaerobik Rumen Fungus Caecomyces sp. GMLF12’nin Linoleate Isomerase Enzim Aktivitesinin Belirlenmesi}, journal={KSÜ Doğa Bilimleri Dergisi}, volume={14}, pages={12–17}, year={2012}, DOI={10.18016/ksujns.13858}, author={Çömlekçioğlu, Uğur and Özköse, Emin and Kar, Bülent and Akyol, İsmail and Ekinci, M.}, abstract={<p> <span style="font-size: 12pt; font-family: "Times New Roman","serif";"> <strong>Özet: </strong> Bu çalışmada anaerobik rumen fungusu <em>Caecomyces </em> sp. GMLF12’de linoleate isomerase enziminin varlığı araştırılmıştır. Linoleate isomerase enzimi linoleik asitin konjuge linoleik asite izomerizasyonunu sağlamaktadır. Farklı konsantrasyonlarda linoleik asit içeren besi ortamına inokule edilen <em>Caecomyces </em> sp. ve <em>Neocallimastix </em> sp. izolatlarının gelişmediği görülmüştür. Bunun üzerine glikozlu besi ortamında geliştirilmiş <em>Caecomyces </em> sp. GMLF12’nin küresel rizoidleri enzim kaynağı olarak kullanılmıştır. Hücreler, linoleik asit ile muamele edilmiş ve belirli zaman aralıklarında enzimatik reaksiyon durdurularak KLA düzeyleri spektrofotometrik olarak ölçülmüştür. En yüksek KLA düzeyi 45. dakika sonunda 8.22 µg/ml olarak ölçülmüştür. Elde edilen sonuçlar <em>Caecomyces </em> sp. GMLF12’nin linoleate isomerase enzimine sahip olduğu ve bu enzimin hücre yüzeyinde bulunduğunu göstermiştir. </span> </p> <p> <p class="MsoNormal" style="text-align: justify;">  </p> <p class="MsoNormal" style="text-align: justify;"> <strong style="mso-bidi-font-weight: normal;"> <span style="font-size: 10.0pt;" lang="TR">Anahtar Sözcükler: </span> </strong> <span style="font-size: 10.0pt;" lang="TR"> Rumen, Caecomyces, Biyohidrojenasyon, Linoleat izomeraz, Konjuge Linoleik Asit </span> </p> </p> <p> <p class="MsoNormal" style="text-align: justify;"> <strong style="mso-bidi-font-weight: normal;"> <span style="font-size: 10.0pt;" lang="TR">  </span> </strong> <strong style="mso-bidi-font-weight: normal;"> <span style="font-size: 10.0pt;" lang="TR">Determination of Linoleate Isomerase Enzyme Activity of Anaerobic Rumen Fungus <em style="mso-bidi-font-style: normal;">Caecomyces </em> sp. GMLF12 </span> </strong> </p> <p class="MsoNormal" style="text-align: justify;"> <strong style="mso-bidi-font-weight: normal;"> <span style="font-size: 10.0pt;" lang="TR">  </span> </strong> </p> <p class="MsoNormal" style="text-align: justify;"> <strong style="mso-bidi-font-weight: normal;"> <span style="font-size: 10.0pt;" lang="TR">ABSTRACT: </span> </strong> <span style="font-size: 10.0pt; mso-ansi-language: EN-US;">In this study, the presence of linoleate isomerase enzyme was investigated in <em style="mso-bidi-font-style: normal;">Caecomyces </em>sp. GMLF12. Linoleate isomerase enzyme enables the isomerisation of linoleic acid to conjugated linoleic acid. <em style="mso-bidi-font-style: normal;">Caecomyces </em> sp. GMLF12 isolate was inoculated to different concentrations of linoleic acid-containing media, but the fungi were not developed. Upon this, <em style="mso-bidi-font-style: normal;">Caecomyces </em> sp. GMLF12 was grown in glucose containing medium and spherical rhizoids were used as enzyme source. Cells were treated with linoleic acid, and enzymatic reaction was halted at given time intervals and CLA levels were measured by spectrophotometrically. The maximum CLA level was measured as 8.22 µg/ml at 45. min. The results showed that <em style="mso-bidi-font-style: normal;">Caecomyces </em> sp. GMLF12 have linoleate isomerase enzyme and this enzyme was found in cell surface. </span> </p> <p class="MsoNormal" style="text-align: justify;"> <strong style="mso-bidi-font-weight: normal;"> <span style="font-size: 10.0pt;" lang="TR">Key Words: </span> </strong> <span style="font-size: 10.0pt;" lang="TR"> Rumen, <em style="mso-bidi-font-style: normal;">Caecomyces </em>, Biohidrogenation, Linoleate isomerase, Conjugated Linoleic Acid </span> </p> <span style="font-size: 12pt; font-family: "Times New Roman","serif";">  </span> </p>}, number={4}, publisher={Kahramanmaraş Sütçü İmam Üniversitesi}