TY - JOUR TT - Comparative analysis of microsatellite loci in chicken and pigeon AU - Öz, Gülseren Yıldız AU - Çakır, Sema AU - Kurhan, İsmail AU - Doğan, Müge AU - Şahin, Elif AU - Nizamlıoğlu, Mehmet AU - Kurar, Ercan PY - 2015 DA - March JF - Eurasian Journal of Veterinary Sciences JO - Eurasian J Vet Sci PB - Selçuk Üniversitesi WT - DergiPark SN - 1309-6958 SP - 43 EP - 50 VL - 31 IS - 1 KW - Güvercin KW - tavuk KW - mikrosatellit markör KW - polimorfizm N2 - Aim: The aim of this study is to investigate utilization of chicken microsatellite loci in genetic studies of pigeon (Columba livia) populations.Materials and Methods: A total of chicken 100 microsatellite markers were selected from chicken linkage maps. DNAs of pigeons and a chicken as a positive control were used to amplify specific genomic regions by polymerase chain reaction (PCR). The resulting PCR products were separated by capillary electrophoresis and allele genotypes were determined. In a pilot study, general population parameters including number of allele (Na), observed (Ho) and expected (He) heterozygosities and deviation from Hardy-Weinberg Equilibrium (HWE) were calculated for 24 positive loci in a pigeon population.Results: A total of 48 microsatellite loci were amplified (48%) in pigeon DNA and expected PCR products were observed. In the pilot study, alleles numbers varied 1-5 and mean Na was 2.5 for 24 loci. A total of 60 different alleles were determined. Ho and He values were observed as 0.0001.000 and 0.000-0.698, respectively.Conclusion: Although Na and polymorphism levels were lower in general, results of this study suggested that chicken microsatellite loci can be used in genetic studies of pigeons. CR - Ando H, Kaneko S, Suzuki H, et al., 2011. Genetic diversity of the Japanese Wood Pigeon, Columba janthina, endemic to islands of east Asia, estimated by newly developed microsatellite markers. Zool Sci, 28, 891-896. 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UR - https://dergipark.org.tr/tr/pub/eurasianjvetsci/issue//261977 L1 - https://dergipark.org.tr/tr/download/article-file/228497 ER -