TY  - JOUR
TT  - Phenotyping determination of CYP1A2 enzyme activity using caffeine in sheep
AU  - Uney, Kamil
AU  - Tras, Bünyamin
PY  - 2011
DA  - March
JF  - Eurasian Journal of Veterinary Sciences
JO  - Eurasian J Vet Sci
PB  - Selçuk Üniversitesi
WT  - DergiPark
SN  - 1309-6958
SP  - 27
EP  - 32
VL  - 27
IS  - 1
KW  - Kafein
KW  - CYP1A2 aktivitesi
KW  - koyun
KW  - ırk
N2  - Aim: The aims of this study were to determine the validity of the plasma metabolic ratios (MR) to investigate the CYP1A2 activity in practice and to compare in vivo CYP1A2 enzyme activity using caffeine (CF) as a probe in Morkaraman (MK), Akkaraman (AK) and Anatolia Merino (AM) sheep breeds.Materials and Methods: Caffeine was administered as a single dose of 5 mg/kg b.w. by the intravenous in MK, AK and AM sheep breeds. The plasma levels of CF and paraxanthine (PX) were measured using high-performance liquid chromatography. CYP1A2 phenotyping was measured using the ratio [(PX/CF)concentration-time curve (AUCs) of PX and CF and the ratios [(PX/CF)PX and CF at 3 to 16 h after CF administration. Correlations between the plasma (PX/CF)were determined to investigate of the CYP1A2 phenotyping by single blood sampling in practice.
UR  - https://dergipark.org.tr/tr/pub/eurasianjvetsci/issue//262166
L1  - https://dergipark.org.tr/tr/download/article-file/228688
ER  -