TY - JOUR TT - Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi AU - Albayrak İskender, Nurcan AU - Örtücü, Serkan AU - Aksu, Yaşar PY - 2017 DA - April Y2 - 2017 DO - 10.17474/artvinofd.274071 JF - Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi JO - AÇÜOFD PB - Artvin Çoruh Üniversitesi WT - DergiPark SN - 2146-1880 SP - 55 EP - 61 VL - 18 IS - 1 KW - Hyphantria cunea KW - bacteria KW - biological control KW - pest KW - pathogenicity N2 - Amerikan Beyaz Kelebeği, Hyphantriacunea (Drury) sayısız konukçubitkisi olan polifag bir zararlıdır. Buçalışmada, H. cunea’nınbakteriyal florası zararlıya karşı mikrobiyal kontrol ajanı olabilecek yeniorganizmaları identifiye etmek için araştırıldı. H. cunea’danaltı bakteri izole edilerek saflaştırıldı. Bakteriyal izolatların morfolojik,biyokimyasal, diğer fenotipik özellikleri (API 20E, API 50 CH,API Staph ve API Coryne)belirlendi. İlave olarak, 16S rRNA gen sekansanalizi de yapıldı. Yapılan çalışmalar sonucunda izolatlar; Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4),Kocuria palustris (Abk6), Arthrobacterarilaitensis (Abk7)ve Microbacterium oxydans (Abk8)olarak tanımlandı. Bütün bakteriler H. cunea’nınüçüncü-dördüncü gömlek larvalarına karşı oniki gün boyunca test edildi. Enyüksek insektisidal aktivite %30 ile L. sphaericus (Abk1)’dan elde edildi (p<0.05). Bu sonuçlar, L. sphaericus (Abk1)’un, H.cunea’nın mikrobiyal mücadelesinde dikkatealınabileceğini göstermiştir. Gelecekte, L. sphaericus (Abk1)’un patojenite zenginleştirme stratejileri(diğer entomopatojelerle veya insektisitlerle kombine edilmesi gibi)kullanılarak H. cunea üzerindekietkinliğinin artırılması yönünde çalışmalar yapılacaktır. CR - Abbott WS (1925) A method of computing the effectiveness of aninsecticide. J Econ Entomol 18: 265-267. CR - Albayrak Iskender N, Örtücü S, Aksu Y (2012). 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UR - https://doi.org/10.17474/artvinofd.274071 L1 - https://dergipark.org.tr/tr/download/article-file/280864 ER -