TY  - JOUR
TT  - IN VITRO CYTOTOXICITY AND APOPTOTIC PROFILE OF RHEUM RIBES
AU  - Erdoğan, Mehmet Kadir
PY  - 2017
DA  - February
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 126
EP  - 126
VL  - Volume 2
IS  - İssue 1 (1)
KW  - IN VITRO CYTOTOXICITY AND APOPTOTIC PROFILE OF RHEUM RIBES
N2  - Rheum speciesare medicinally important plants due to the presence of anthracene derivativesoccurring in the subterranean parts of the plant. Rheum ribes L. (Polygonaceae)is the source of one of the most important crude drugs in the Middle East,roots are used as oriental laxative medicine and an antipsoriatic drug. R.ribes is locally known as ‘‘ışkın, uçgun or, uçgun’’ and grown mostly in EasternTurkey, Lebanon and Iran.The aim of thisstudy was to investigate the mechanism by which Rheum ribes inducesantiprolif-erative and apoptotic effects in HT 29 human colorectal carcinomacells. Dose and time dependent cytotoxic effects of Rheum ribes were evaluatedby the 3 (4,5 dimethylthiazol 2 y1) 2,5 diphenyltetrazolium bromide (MTT)assay. Cell Proliferation Kit I (MTT) was purchased from Roche (RocheDiagnostics, Mannheim, Germany). Briefly, HT 29 cells were seeded at a 1×104concentration in 96 well plates overnight and then treated with 10 µl MTTlabeling reagent. After 4 h, 100 µl of the Solubilization solution was addedinto each well and incubated 24 h in a humidified atmosphere (+37 °C, 5-6%CO2). Absorbance of the formazan product was measured at 550 and 600 nmwavelenght by Elisa microplate reader. Results are expressed as a percentage ofthe untreated controls. Cell Death Detection Elisa Kit (Roche) was used to detect apoptoticeffect of Rheum ribes, according to the manufacturer’s protocol. Additionally,propidium iodide (50 µg/mL) and ethidium bromide (50 µg/mL) were used fordetecting the apoptotic cells. Results were evaluated comparatively andstatistically.
CR  - Mehmet Kadir ERDOĞAN
Department of Biology, Faculty of Arts and Sciences, Bingöl University, 12000, Bingöl, Turkey
UR  - https://dergipark.org.tr/tr/pub/turjoem/issue//305886
L1  - https://dergipark.org.tr/tr/download/article-file/293546
ER  -