TY  - JOUR
TT  - EVALUATION OF GENOTOXIC AND ANTIGENOTOXIC EFFECTS OF PUERARIN BY MICRONUCLEUS ASSAY ON MAMMALIAN CELL LINES
AU  - Anlar, Hatice Gül
AU  - Bacanlı, Merve
AU  - Başaran, A. Ahmet
AU  - Başaran, Nurşen
PY  - 2017
DA  - February
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 129
EP  - 129
VL  - Volume 2
IS  - İssue 1 (1)
KW  - EVALUATION OF GENOTOXIC AND ANTIGENOTOXIC EFFECTS OF PUERARIN BY MICRONUCLEUS ASSAY ON MAMMALIAN CELL LINES
N2  - It is well knownthat free oxygen radicals play important role in the pathogenesis of chronicdisorders such as cancer, diabetes, cardiovascular and neurological diseases.Recent interest in plant phenolics has increased because of their protectiveeffects against free oxygen radicals. Puerarin[7-hydroxy-3-(4-hydroxyphenyl)-1-benzopyran-4-one 8-(β-D-glucopyranozid)],isoflavone glycoside, primarily derived from Ge-gen (Pueraria lobata, RadixPuerariae). This medicinal plant is commonly used in Chinese folk medicine.Pueraria lobata has been used against alcohol dependency, hypertension, fever,headache, diarrhea and eye disorders. Phytoestrogenic activity of puerarin dueto its isoflavone structure is very important in woman diseases. In the presentstudy genotoxic/antigenotoxic effects of puerarin were assessed by micronucleus(MN) assay in human peripheral blood lymphocytes and Chinese hamster lungfibroblast cell line (V79). In MN assay, the cells were treated with 1, 5, 10,25 and 50 µM concentrations of puerarin. Hydrogen peroxide (H2O2), 50 µM, wasused as positive control and 1% DMSO was used as negative control. In ourstudy, the cells were treated with different concentrations of puerarin causedno genotoxic effects alone at all studied concentrations as compared with thenegative control. MN frequencies of puerarin treated cells were found to bedecreased when compared to positive control. It seems that puerarin might havea role in the prevention of genotoxic damage.
CR  - Hatice Gül ANLAR, Merve BACANLI, A. Ahmet BAŞARAN, Nurşen BAŞARAN
CR  - Hacettepe University Faculty of Pharmacy Department of Pharmaceutical Toxicology 06100 Ankara, Turkey
Çukurova University Faculty of Pharmacy Department of Pharmaceutical Toxicology 06330 Adana, Turkey
Hacettepe University Faculty of Pharmacy Department of Pharmacognosy 06100 Ankara, Turkey
UR  - https://dergipark.org.tr/tr/pub/turjoem/issue//305890
L1  - https://dergipark.org.tr/tr/download/article-file/293552
ER  -