TY  - JOUR
TT  - GENOTOXIC EFFECTS OF COBALT NANOPARTICLES AND IONIC FORM IN DROSOPHILA
AU  - Güneş, Merve
AU  - Yalçın, Burçin
AU  - Ertuğrul, Havva
AU  - Kaya, Bülent
PY  - 2017
DA  - February
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 165
EP  - 165
VL  - Volume 2
IS  - İssue 1 (1)
KW  - GENOTOXIC EFFECTS OF COBALT NANOPARTICLES AND IONIC FORM IN DROSOPHILA
N2  - Nanoparticleswidely used in different industrial areas because of their many advantages.However, there has been little known about toxic effects of NPs on human andenvironment. Today its known that most of diseases associated with mutation,induced recombination and DNA damage. Therefore, determination of such effectsof NPs is important. In this study,the genotoxic effects of CoCl2 and CoNPs were investigated by using bothSomatic Mutation and Recombination Test (SMART) and Single Cell GelElectrophoresis (COMET) in Drosophila. SMART is reliable assay to detect a widerange of genetic alterations in a rapid and inexpensive way. In SMART test, theeffects of these chemicals were evaluated according to genetic changes (pointmutation, deletion, non-disjunction and recombination) in wing imaginal disccells that lead to the formation of mutant trichomes. On the other hand, AlkalineCOMET assay is fast, powerfull and sensitive test to detect the single strandbreaks in alkali labile lesions individual cells in DNA.CoCl2 showed thepositive results for small single spots, large single spot, total mwh spots andtotal spots categories in only 10 mM concentration in trans-heterozygous flies(mwh/flr3) while CoNP demonstrated positive results for same categories in 1and 10 mM concentrations. Both CoCl2 and CoNP concentrations (0,1, 1 ve 10 mM)significant induced the damage for tail moment and tail lenght. Finally, usedchemicals which in this investigation observed in both COMET and SMART assaycause genotoxic potential. 
CR  - Merve GÜNEŞ, Burçin YALÇIN, Havva ERTUĞRUL, Bülent KAYA
CR  - Akdeniz University, Faculty of Sciences, Biology Department, Antalya
UR  - https://dergipark.org.tr/tr/pub/turjoem/issue//306127
L1  - https://dergipark.org.tr/tr/download/article-file/293881
ER  -