TY  - JOUR
TT  - GENOTOXIC EFFECTS OF SILICON DIOXIDE NANOPARTICLES IN ALLIUM CEPA
AU  - Çalbay, Özlem
AU  - Ünal, Fatma
AU  - Suludere, Zekiye
AU  - Yüzbaşıoğlu, Deniz
PY  - 2017
DA  - February
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 189
EP  - 189
VL  - Volume 2
IS  - İssue 1 (1)
KW  - GENOTOXIC EFFECTS OF SILICON DIOXIDE NANOPARTICLES IN ALLIUM CEPA
N2  - Silicon dioxidenanoparticles-SiO2 NPs, are widely used in many nanotechnological products. Sothe production, usage, and release of these nanoparticles to the environmentare increasing every day. It is reported that nanoparticles may have toxic,particularly genotoxic effects. The aim of this study was to investigate thegenotoxic potential of SiO2 NPs by using root cells of Allium cepa which is anindicator organism. Roots were treated with 50, 250, 500 and 1000 μg/ml for 24,48 and 72 h. SiO2 NPs decreased the mitotic index in Allium cepa however, thisdecrease was significant at 50 and 1000 μg/ml at 24 h, 1000 μg/ml at 48 h, and50 μg/ml at 72 h treatment, compared to the control. Moreover, SiO2 NPsincreased the frequency of chromosomal abnormalities at all the concentrationsand treatment times compared to the control. C-metaphase, sticky chromosomes,disturbed pro-meta-ana-telophase, chromosomal break, asynchron division,multipolarity, lagging and vagrant chromosomes, star anaphase, micronuclei, andloss of genetic material were observed under light microscopy. Transmissionelectron microscopy analyses showed internalization of NPs inside the cell. Onentering the cells, the nanoparticles are transported from one cell to otherthrough plasmadesmata. NPs can reach to the cell wall, cytoplasm, cell nucleus,mitochondria and some other structures like vacuole. These observations areverified by the cytotoxic and clastogenic/genotoxic effect of SiO2 NPs detectedby light microscopy. These findings also suggest that Allium cepa can be usedfor the genotoxicity monitoring of novel nanomaterials that are used in manyconsumer products. 
CR  - Özlem ÇALBAY, Fatma ÜNAL, Zekiye SULUDERE, Deniz YÜZBAŞIOĞLU
CR  - Gazi University, Science Faculty, Department of Biology, Genetic Toxicology Laboratory, 06500, Ankara, TURKEY
UR  - https://dergipark.org.tr/tr/pub/turjoem/issue//306329
L1  - https://dergipark.org.tr/tr/download/article-file/294134
ER  -