TY  - JOUR
TT  - HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) OVARY TISSUE
AU  - Yön, Nazan Deniz
AU  - Öztürk, Ceyda
AU  - Zengin, Mustafa
AU  - Akbulut, Cansu
PY  - 2017
DA  - February
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 205
EP  - 205
VL  - Volume 2
IS  - İssue 1 (1)
KW  - HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) OVARY TISSUE
N2  - Examination the histopathologicaleffects of (4s)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic acid onovary tissue of zebrafish were aimed. (4S)-2-(4-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid is new synthesized substance which obtained fromcysteine and valine. Because of thiazolidine derivates have importantbiological responses scientist work intensively on these compounds recentyears. It is obvious that thiazolidine contained compounds will be used infuture in the pharmaceutical industry to treat important diseases.Zebrafish were raised in acomputer-controlled incubation chamber, and received 14 hours of daylight and10 hours of darkness everyday. After one week adaptation period zebrafishdivided into four group (n=10) as one control and experimental groups (0,2mM, 0,4mM,0,6Mm). For investigating the effects of (4s)-2-(4-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid , ovary tissues were dissected after 5 day ofthe exposure. Tissues were fixed with 10% neutral buffered formalin anddehydration were carried out in an ascending series of ethanol. After tissueswere cleared in xylene, embedded in paraffin wax and cut into 5 µm sections ona microtome. The sections were stained with hematoxylin (H&E). Results wereevaluated with light microscope.In control group normal ovaryhistology was observed. Primary oocytes, cortical alveolus stage oocytes,vitellogenic oocytes and mature oocytes were monitored clearly. In 0,2mMexposure group, seperation between follicular epithelium and zona radiata wasalso monitored at cortical alveoli. Opening at structure of oocytes wereobserved. Openings between zona radiata and vitelline envelope were detected atcortical alveoli. Disintegration of vitelline envelope were monitored atvitellogenic oocyte structure. Vacuolization were observed at cortical alveolusstage oocyte structure. In 0,4mM exposure group, seperation between follicularepithelium and zona radiata was also monitored at mature oocyte. Vacuolizationat cortical alveolus stage oocytes were monitored. Seperation betweenfollicular epithelium and zona radiata was monitored at cortical alveoli. In0,6mM exposure group, openings between zona radiata and vitelline envelope weredetected at cortical alveoli. Vacuolization at cortical alveolus stage oocyteswere monitored. Degeneration was detected at mature oocyte.
CR  - Nazan Deniz YÖN, Ceyda ÖZTÜRK, Mustafa ZENGİN, Cansu AKBULUT
CR  - Sakarya University Science and Letters Faculty Department of Biology,  Sakarya, Turkey
Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Turkey
UR  - https://dergipark.org.tr/tr/pub/turjoem/article/306607
L1  - https://dergipark.org.tr/tr/download/article-file/294508
ER  -