@article{article_317364, title={Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat}, journal={Harran University Journal of the Faculty of Veterinary Medicine}, volume={5}, pages={124–128}, year={2016}, author={Anadol, Elvan and Yarım, Gül Fatma and Gültiken, Nilgün and Kazak, Filiz}, keywords={Oksidatif stres, Ovaryohisterektomi, Rat}, abstract={The purpose of the present study was to evaluate the effect of ovariohysterectomy (OHE) on oxidative stress in rats. A total of 12 female Sprague Dawley rats weighing 200-250 g were included in the study. Six rats underwent OHE (group I) and the other 6 rats sham operation (group II) under xylazine (5 mg/kg, i.m.) and ketamine (45 mg/kg, i.m.) anesthesia. In both groups, blood samples were collected preoperatively, day 1 and 10th days after OHE. Serum concentration of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured by spectrophotometric methods. The concentration of MDA increased while activities of SOD and GPx decreased in group I compared to group II on day 1 after the surgery. Similarly, the concentration of MDA was higher while activities of SOD and GPx appeared to be lower in group I than that of group II, although the differences were not statistically significant on the 10th day after surgery. In conclusion, our findings indicated that ovariohysterectomy is related with oxidative stress in the rat.}, number={2}, publisher={Harran Üniversitesi}