TY - JOUR TT - MICROSATELLITE AND ALLOZYME VARIATIONS IN STARLET STURGEON WILD BROODSTOCK AND HATCHERY-PRODUCED OFFSPRING, USED FOR RESTOCKING OF LOWER DANUBE RIVER AU - Ivanova, Petya P. AU - Dzhembekova, Nina S. AU - Kardjeva, Velichka AU - Tsekov, Angel G. AU - Raykov, Violin S. PY - 2017 DA - October DO - 10.3153/JAEFR17022 JF - Journal of Aquaculture Engineering and Fisheries Research JO - J Aquacult Eng Fish Res PB - Özkan ÖZDEN WT - DergiPark SN - 2149-0236 SP - 199 EP - 206 VL - 3 IS - 4 KW - Sterlet KW - Microsatellite KW - Allozymes KW - Identi-fication KW - Restocking N2 - The naturalpopulation of all sturgeon species has been seriously affected byoverexploitation in combination with a substantial loss and degradation ofhabitats during the 20th century. Fish species undergoing torestocking require information about the genetic identity of the existing fishpopulations. The genetic pattern of sterlet (broodstock and offspring) wereanalyzed, using allozymes and seven microsatellite loci (LS19, LS34, LS39,LS54, LS68, Aox45 and Aox27) as markers for species identification. From theseven microsatellite loci three (LS57, Aox23 and Aox45) have shown a tetrasomicprofile. The most polymorphic locus for the broodstock was LS-57 with 12alleles in population, followed by Aox45, LS-68 and LS-54 with 10 alleles. For offspring,the most polymorphic locus was Aox 23 with 11 alleles. Other loci presented a lowerlevel of polymorphism range between 2 to 8 alleles identified for the analyzedindividuals. The average observed and expected heterozygosities were 0.429 and0.413 in the broodstock and 0.500 and 0.423 in the offspring, respectively. Thepreliminary data showed that selected allozymes and microsatellite markersallow identification of the stellate sturgeon from Bulgarian farms and could be applied to test and control the broodstocks used for restocking programmes. 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