TY - JOUR TT - Alternative Method Study for Determining Bactericidal Efficacy of Antimicrobial Paints AU - Çalışkan Özdemir, Sennur AU - Halat, Ece AU - Şahiner, Aslı PY - 2017 DA - February JF - The Turkish Journal Of Occupational / Environmental Medicine and Safety JO - turjoem PB - Engin TUTKUN WT - DergiPark SN - 2149-4711 SP - 29 EP - 29 VL - Volume 2 IS - Issue 1(2) KW - Alternative Method Study for Determining Bactericidal Efficacy of Antimicrobial Paints N2 - Antimicrobialpaints contain various active substances to inhibit or reduce growth of microorganisms.Interior and exterior wall paints with antimicrobial substances developed inrecent years started to be used in several areas with high infection riskprimarily in hospitals. In this study wall paints with 4 different activesubstances from market were tested against S. aureus (ATCC 6538) and E.coli(ATCC 10536) to determine and compare the bactericidal efficacy according toISO 22196. Within the scope of this study, cellulose filter papers were cut 5 x5 cm of size and painted with antimicrobial paints. Paint without antimicrobialsubstances were used as control for this test. Painted filter papers were leftto dry for one week. Painted filter papers and film coatings were exposed totwo sided UV sterilization. Suspension was prepared from 24 h bacterialcultures with 105 cfu/ml concentration. 100 μl of bacterial suspensions wereinoculated on painted surfaces. After inoculation, surfaces were coated withsterile parafilms. After 1, 3, 6 and 24 hour contact times in 37°C, filterpapers were transferred to bottles filled with SCDLP broth. Serial dilutionswere prepared and inoculations were made through pour plating method. Petridishes were incubated in 37°C for 24 h. Bactericidal efficacy of 4 differentactive substances and 4 different contact times were determined. Paint with0.10% zinc prythion provided 99.2% reduction against S.aureus at the 3rd hour,all other active substances provided 99% reduction at 6th hour. Paint with 0.2% AgCl provided 99.98% reduction against E.coli at the 3rd hour. In conclusion,this study indicates that antibacterial efficacy of 4 antibacterial paints withdifferent active substances against E.coli and S.aureus start to increase after6th hour. It was also observed that shorter contact time was effective with zincprythionon Gr (+), AgCl on Gr (-).     Keywords: Antimicrobial paint, bactericidal efficacy, Zinc prythion,Silver chloride CR - Sennur Çalışkan Özdemir, Ece Halat, Aslı Şahiner Ege University, Faculty of Science, Department of Biology, Izmir, Turkey UR - https://dergipark.org.tr/tr/pub/turjoem/issue//319666 L1 - https://dergipark.org.tr/tr/download/article-file/311721 ER -