TY  - JOUR
TT  - Molecular identification of mosquito species using DNA barcode analysis
AU  - Guz, Nurper
AU  - Durmuşoğlu, Enver
PY  - 2017
DA  - February
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 48
EP  - 48
VL  - Volume 2
IS  - Issue 1(2)
KW  - Molecular identification of mosquito species using DNA barcode analysis
N2  - Mosquitoes are a key threat to public health since they areinvolved in transmission of devastating pathogens and parasites. The currentclassification of these vectors is based on morphological characters which havemany drawbacks such as being time consuming and error-prone due to variation ofindividual taxonomic expertise of the analysts as well as phenotypicalvariations. One of the most serious drawbacks of only using this approach forspecies identification is the fact that some traits or characters are apparentonly during certain life cycle stages or in one gender. Therefore, DNA-basedapproaches can overcome many of the aforesaid problems. In this study weanalyzed immature mosquito specimens collected from different habitats of MuğlaProvince, Turkey. A 658 base pair (bp) region of the cytochrome c oxidase (COI)gene used as a universal marker in order to identify mosquito species. Analyzednucleotide sequences were found without pseudo genes and indels that match withhigh similarity to the mosquito sequences in NCBI database. The most common andabundant mosquito species were found to be Culex and Culiseta species.Individuals of the same species grouped closely together in aNeighborhood-Joining (NJ) tree with Kimura 2-parameter (K2P) distances based onCOI sequence similarity. In conclusion COI gene based DNA barcoding is areliable and standardized identification system for most mosquito species andfor studies at the population level.      Keywords: Cytochrome c oxidase, DNA barcoding,Mosquitoes, Phylogenetic analysis
CR  - Nurper Guz, Enver Durmuşoğlu
CR  - Ankara University, Insect Molecular Biology Laboratory, Department of Plant Protection, Faculty of Agriculture, Ankara, Turkey
Ege University, Department of Plant Protection, Faculty of Agriculture, İzmir, Turkey
UR  - https://dergipark.org.tr/tr/pub/turjoem/issue//320469
L1  - https://dergipark.org.tr/tr/download/article-file/313078
ER  -