@article{article_325735, title={A Comparison of the Performance of Exfoliative Cytopathology, Polymerase Chain Reaction (PCR), Culture and ELISA in the Detection of Brucella canis}, journal={Harran University Journal of the Faculty of Veterinary Medicine}, volume={6}, pages={51–56}, year={2017}, DOI={10.31196/huvfd.325735}, author={Yumusak, Nihat and Polat, Pelin Fatos and Erdenlıg Gurbılek, Sevil and Cebı Sen, Cigdem and Yılmaz, Rahsan}, keywords={Brusella canis, Sitopatoloji, PCR, ELISA.}, abstract={<p class="MsoNormal" style="text-align:justify;"> <span lang="en-gb" style="font-size:9pt;font-family:Calibri, ’sans-serif’;" xml:lang="en-gb"> <span style="font-size:12px;">This study was aimed to determine the performance of
cytopathology for the diagnosis of </span> <i> <span style="font-size:12px;">B.
canis </span> </i> <span style="font-size:12px;">on the basis of a comparison with culture, PCR and ELISA results. For
this purpose, swabs for cytological, molecular and bacteriological examinations
blood serum samples for serological tests were taken from 147 dogs which are
suspected to have genital infection.. While the cytological samples were
stained with May-Grünwald Giemsa solution, the swab samples were tested by
bacteriologically and PCR and the serum samples were assayed by ELISA.
According to the results, while ELISA yielded a seropositivity rate of 11.5%,
PCR demonstrated a positivity rate of 1.8%. No </span> <i> <span style="font-size:12px;">B. canis </span> </i> <span style="font-size:12px;">could be isolated from the samples were taken for
bacteriological examinations. At cytological examination, it was observed that
the samples contained coccobacilli presented with abundant neutrophil
leukocytes and macrophages. Some of these immune cells and epithelial cells
were observed to contain round/oval shaped bacteria in their cytoplasm. The
results of this study suggest that cytopathology can be used for auxiliary test
for the diagnosis of brucellosis. </span> </span> </p>}, number={1}, publisher={Harran Üniversitesi}