@article{article_333633, title={Effect of Hardaliye on FoxM1 Gene Expression Level of HT-29, DU-145, HeLa Cancer Cells and CF-1 (Mouse Embryonic Fibroblast)}, journal={Akademik Gıda}, volume={15}, pages={119–123}, year={2017}, DOI={10.24323/akademik-gida.333633}, author={Kahraman Ilıkkan, Özge}, keywords={FoxM1, qRT-PCR, Kanser hücre hatları, CF-1, Gen ekspresyonu}, abstract={<p> <span style="font-size:9.5pt;line-height:107%;font-family:Arial, ’sans-serif’;">Chemotherapeutic agents may influence both cancer cells and healthy
cells, therefore alternative therapeutic agents or targets have become a milestone of cancer cure. Forkhead box M1 (FoxM1)
is a transcriptional regulator and a novel cancer therapy target as
overexpressed in cancer cells, and its inhibitors are considered as potential
therapeutic agents to halt cancer progression. Therefore, in this study, the
effect of “Hardaliye” on cancer cells and
a healthy cell line was primarily investigated
by MTT (3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide) and later
on, FoxM1 gene expression levels were determined. Hardaliye, especially when diluted ten and twenty-fold, decreased
viability percentage of all cancer cells and did not affect healthy cells.
FoxM1 levels of cancer cells drastically decreased especially in HT-29 cells
while did not statistically change their levels in the healthy cell line. </span> <br /> </p>}, number={2}, publisher={Sidas Medya Ltd. Şti.}