TY - JOUR TT - Determination of LC50 Value of Borax (Na2O2B2O3.10H2O) on Tenebrio mollitor (Coleoptera: Tenebrionidae) and Determination Toxic Effects of Sublethal Doses of Borax on Mealworm Larvae AU - Eskin, Ata AU - Kocabayraktar, Gülhan AU - Orak, Muhammed AU - Sert, Ayşe AU - Candan, Mehmet AU - Deniz, Gizem PY - 2017 DA - December Y2 - 2017 DO - 10.17100/nevbiltek.334441 JF - Nevşehir Bilim ve Teknoloji Dergisi PB - Nevşehir Hacı Bektaş Veli Üniversitesi WT - DergiPark SN - 2148-466X SP - 467 EP - 475 VL - 6 IS - 2 KW - Giemsa KW - Tenebrio mollitor KW - Hemosit KW - Larval ağırlık KW - Tenebrio mollitor N2 - In this study, lethal concentration 50 (LC50) value of borax on Tenebrio mollitor (Coleoptera: Tenebrionidae) was determined with testing 100, 1.000, 10.000, 100.000 ppm borax doses. The LC50 value was determined after 20 days later as 3.402,15 ppm for larvae and 28.512,5 ppm for adults. Also sublethal 100, 1.000, 3.000 ppm borax doses were tested to determine the effects on the larval weight of insect and the adverse effects on the hemocytes. When at other doses except 100 ppm, a decrease was observed in the larval weight means compared to the control group, also a statistically significant decrease was observed in the 3.000 ppm test group compared to the control group. Finally, apoptosis, necrosis and micronucleus formations were determined by Giemsa staining technique in hemocytes of groups exposed to high doses (1.000 ppm and 3.000 ppm borax). CR - [1] Collins, L., Edwards, L., “Toxicity of moist snuff and impact on various stages of darkling beetles (Tenebrio molitor)”, Explorations, Biology and Biotechnology, 2017. [2] Berger, J., Slavickova, K., “Morphological characterization of hemocytes in the adult Linden Bug, Pyrrhocoris apterus (L.) (Heteroptera), Zoological Studies, 47(4): 466-472, 2008. 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